Team:HKUST-Hong Kong/Notebook/Protocol

From 2012.igem.org

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<body>
<body>
         <div id="Navigation_top">
         <div id="Navigation_top">
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           <div id="iGEM_Logo" class="Upper_Logos"></div>
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           <a href="https://2012.igem.org/Main_Page"><img id="iGEM_Logo" class="Upper_Logos" src="https://static.igem.org/mediawiki/2012/3/32/Igem-logo.png"></a>
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           <div id="HKUST_Logo" class="Upper_Logos"></div>
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           <a href="http://www.ust.hk"><img id="HKUST_Logo" class="Upper_Logos" src=""></a>
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          <a href="https://2012.igem.org/Team:HKUST-Hong_Kong"><img id="HKUST_iGEM_Logo" class="Upper_Logos" src="https://static.igem.org/mediawiki/2012/4/43/295197_446935765350523_472857901_n.jpg"></a>
           <div class="Navigation_Buttons" id="Team" align="center"><h3><p>TEAM</p></h3></div>
           <div class="Navigation_Buttons" id="Team" align="center"><h3><p>TEAM</p></h3></div>
           <div class="Navigation_Buttons" id="Project" align="center"><h3><p>PROJECT</p></h3></div>
           <div class="Navigation_Buttons" id="Project" align="center"><h3><p>PROJECT</p></h3></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Members">Members</a></p></div>
               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Members">Members</a></p></div>
               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Advisors">Advisors</a></p></div>
               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Advisors">Advisors</a></p></div>
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              <div class="Content_Buttons""><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Acknowledgement">Acknowledgement</a></p></div>
 
           </div>
           </div>
           <div class="Navigation_Content" id="Project_Content">
           <div class="Navigation_Content" id="Project_Content">
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Project_Abstraction">Project Abstract</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Project_Abstraction">Abstract</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Background_and_Motive">Background and<br> Motive</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Background_and_Motive">Motive</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Project">Project Description</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Design_Overview">Design - Overview</a></p></div>
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              <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Module">Module</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Design_Module">Design - Module</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Chassis">Chassis</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Design_Chassis">Design - Chassis</a></p></div>
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              <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Safety">Safety</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Expectation">Expectation</a></p></div>
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              <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Potential_Application">Potential applicaton</a></p></div>
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           </div>
           </div>
           <div class="Navigation_Content" id="Wet_lab_Content" align="center">
           <div class="Navigation_Content" id="Wet_lab_Content" align="center">
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Characterization">Characterization</a></p></div>
               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Characterization">Characterization</a></p></div>
               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Achievement">Achievement</a></p></div>
               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Achievement">Achievement</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Prospect">Prospect</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Future_Work">Future Work</a></p></div>
           </div>
           </div>
           <div class="Navigation_Content" id="Human_practice_Content" align="center">
           <div class="Navigation_Content" id="Human_practice_Content" align="center">
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           </div>
           </div>
           <div class="Navigation_Content" id="Extras_Content" align="center">
           <div class="Navigation_Content" id="Extras_Content" align="center">
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                <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Medal_Requirements">Medal Requirements</a></p></div>
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                <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Safety">Safety</a></p></div>
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                <div class="Content_Buttons""><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Attribution">Attribution</a></p></div>
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                <div class="Content_Buttons""><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Acknowledgement">Acknowledgement</a></p></div>
               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Glossary">Glossary</a></p></div>
               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Glossary">Glossary</a></p></div>
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              <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Site_Map">Site Map</a></p></div>
 
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              <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Photo_Gallery">Photo Gallery</a></p></div>
 
           </div>
           </div>
         </div>
         </div>
           <div><p align="center"><font size="20">PROTOCOL</font></p></div>
           <div><p align="center"><font size="20">PROTOCOL</font></p></div>
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          <div><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Notebook"><<< Return to Notebook</a></p></div>
  <div id="paragraph0" class="bodyParagraphs">
  <div id="paragraph0" class="bodyParagraphs">
           <div align="left">
           <div align="left">
               <h1>General Protocol</h1>
               <h1>General Protocol</h1>
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          </div><ol>
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          <li><p><a href="https://static.igem.org/mediawiki/2012/a/a4/Iodine_test_protocol.pdf" target="_blank">Amylase Test for Integration of <i>Bacillus subtilis</i></a></p></li>
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          <li><p><a href="https://static.igem.org/mediawiki/2012/6/6a/Transformation_of_Bacillus_subtilus.pdf" target="_blank">Transformation of Integration Vector into <i>Bacillus subtilis</i></a></p></li></ol>
           </div>
           </div>
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          <p><a href="https://static.igem.org/mediawiki/2012/a/a4/Iodine_test_protocol.pdf">Iodine Test Protocol</a></p>
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          </div>
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  <div id="paragraph1" class="bodyParagraphs">
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           <div align="left">
           <div align="left">
               <h1>Target Binding</h1>
               <h1>Target Binding</h1>
           </div>
           </div>
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<ol>
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          <li><p><a href="https://static.igem.org/mediawiki/2012/e/e7/HKUST_K733007_Specific_Protocol.pdf" target="_blank">BBa_K733007 construct protocol</a></p></li>
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          <li><p><a href="https://static.igem.org/mediawiki/2012/7/7d/K733008_Specific_Protocol.pdf" target="_blank">BBa_K733008 construct protocol</a></p></li></ol>
           <p></p>
           <p></p>
           </div>
           </div>
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  <div id="paragraph2" class="bodyParagraphs">
  <div id="paragraph2" class="bodyParagraphs">
           <div align="left">
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               <h1>Anti-tumor Molecule secretion</h1>
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               <h1>Anti-tumor Molecule Secretion</h1>
           </div>
           </div>
           <p><ol>
           <p><ol>
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           <li><a href="https://static.igem.org/mediawiki/2012/7/71/BBa_K733005_construct_protocol.pdf">BBa_K733005 construct protocol</a></li><br>
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           <li><a href="https://static.igem.org/mediawiki/2012/7/71/BBa_K733005_construct_protocol.pdf" target="_blank">BBa_K733005 construct protocol</a></li><br>
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           <li><a href="https://static.igem.org/mediawiki/2012/d/d4/BBa_K733006_construct_protocol.pdf">BBa_K733006 construct protocol</a></li><br>
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           <li><a href="https://static.igem.org/mediawiki/2012/d/d4/BBa_K733006_construct_protocol.pdf" target="_blank">BBa_K733006 construct protocol</a></li><br>
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           <li><a href="https://static.igem.org/mediawiki/2012/1/16/BBa_K733013_construct_protocol.pdf">BBa_K733013 construct protocol</a></li><br>
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           <li><a href="https://static.igem.org/mediawiki/2012/1/16/BBa_K733013_construct_protocol.pdf" target="_blank">BBa_K733013 construct protocol</a></li><br>
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           <li><a href="https://static.igem.org/mediawiki/2012/c/c5/BBa_K733014%2B_BBa_K733016_construct_protocol.pdf">BBa_K733014+ BBa_K733016 construct protocol</a></li><br>
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           <li><a href="https://static.igem.org/mediawiki/2012/c/c5/BBa_K733014%2B_BBa_K733016_construct_protocol.pdf" target="_blank">BBa_K733014+ BBa_K733016 construct protocol</a></li><br>
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           <li><a href="https://static.igem.org/mediawiki/2012/a/a7/BBa_K733015%2B_BBa_K733017_construct_protocol.pdf">BBa_K733015+ BBa_K733017 construct protocol</a></li><br>
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           <li><a href="https://static.igem.org/mediawiki/2012/a/a7/BBa_K733015%2B_BBa_K733017_construct_protocol.pdf" target="_blank">BBa_K733015+ BBa_K733017 construct protocol</a></li><br>
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           <li><a href="https://static.igem.org/mediawiki/2012/8/8a/BBa_K733016_transform_in_B.subtilis_protocol.pdf">BBa_K733016 transform in B.subtilis protocol</a></li><br>
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           <li><a href="https://static.igem.org/mediawiki/2012/8/8a/BBa_K733016_transform_in_B.subtilis_protocol.pdf" target="_blank">BBa_K733016 transform in <i>B.subtilis protocol</i></a></li><br>
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           <li><a href="https://static.igem.org/mediawiki/2012/1/1e/BBa_K733017_transform_in_B.subtilis_protocol.pdf">BBa_K733017 transform in B.subtilis protocol</a></li><br>
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           <li><a href="https://static.igem.org/mediawiki/2012/1/1e/BBa_K733017_transform_in_B.subtilis_protocol.pdf" target="_blank">BBa_K733017 transform in <i>B.subtilis</i> protocol</a></li><br>
           </ol></p>
           </ol></p>
           </div>
           </div>
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           <div align="left">
           <div align="left">
               <h1>Regulation and Control System</h1>
               <h1>Regulation and Control System</h1>
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           </div>
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           </div><ol>
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           <p></p>
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<li><p><a href="https://static.igem.org/mediawiki/2012/6/6e/Protocol_for_the_construct_of_Ptms_in_pSB1C3.pdf" target="_blank">BBA_K733001</a></p></li>
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           </div>
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<li><p><a href="https://static.igem.org/mediawiki/2012/7/7e/Protocol_for_the_construct_of_xylR%2BPxylA_in_pSB1C3.pdf" target="_blank">BBA_K733002</a></p></li>
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           <li><p><a href="https://static.igem.org/mediawiki/2012/6/6b/Protocol_for_the_construct_of_RBS%2BydcD_in_pSB1C3.pdf" target="_blank">BBA_K733003</a></p></li>
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           <li><p><a href="https://static.igem.org/mediawiki/2012/c/ca/Protocol_for_the_construct_of_RBS%2BydcE_in_pSB1C3.pdf" target="_blank">BBA_K733004</a></p></li>
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          </ol></div>
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<div id="Sitemap">
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<div id="Sitemap_Home" align="center">
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<p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong"><b>Home</b></a></p>
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</div>
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<div class="Sitemap_Content">
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<li><p><b>Team</b><p><ol>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Introduction">Introduction</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Supervisor">Supervisor</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Instructor">Instructor</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Members">Members</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Advisors">Advisors</a></p></li>
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</ol>
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</div>
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<div class="Sitemap_Content">
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<li><p><b>Project</b></p><ol>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Project_Abstraction">Abstract</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Background_and_Motive">Motive</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Design_Overview">Design - Overview</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Design_Module">Design - Module</a></p></li>
 +
<p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Module/Target_binding">Target Binding Module</a></p>
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<p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Module/Anti_tumor">Anti-tumor Molecule Secretion Module</a></p>
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<p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Module/Regulation_and_control">Regulation and Control Module</a></p>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Design_Chassis">Design - Chassis</a></p></li></ol>
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</div>
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<div class="Sitemap_Content">
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<li><p><b>Wet Lab</b></p><ol>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Parts_and_Device">Parts and Devices</a></p></li>
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<p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Parts_and_Device">Overview</a></p>
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<p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Construction">Construction</a></p>
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<p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Assembly">Assembly</a></p>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Notebook">Notebook</a></p></li>
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<p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Notebook/Logbook">Logbook</a></p>
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<p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Notebook/Protocol">Protocol</a></p>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Characterization">Characterization</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Achievement">Achievement</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Future_Work">Future Work</a></p></li></ol>
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</div>
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<div class="Sitemap_Content">
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<li><p><b>Human Practice</b></p><ol>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Overview">Overview</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Interview">Interview</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Presentation">Presentation</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Calendar">Calendar</a></p></li></ol>
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</div>
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<div class="Sitemap_Content">
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<li><p><b>Extras</b></p><ol>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Medal_Requirements">Medal Requirements</a></p></li>
 +
<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Safety">Safety</a></p></li>
 +
<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Attribution">Attribution</a></p></li>
 +
<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Acknowledgement">Acknowledgement</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Glossary">Glossary</a></p></li></ol>
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</div>
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</div>
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 +
}
 +
.Sitemap_Content{
 +
background-color:#ffffff;
 +
opacity:0.8;
 +
width:173px;
 +
height:auto;
 +
float:left;
 +
margin:1px;
 +
padding-bottom:5px;
 +
padding-left:5px;
 +
border:3px solid #000000;
 +
border-radius:10px;
 +
-moz-border-radius:10px;
 +
}
 +
#Sitemap_Home{
 +
background-color:#ffffff;
 +
opacity:0.8;
 +
width:940px;
 +
height:auto;
 +
float:left;
 +
margin:1px;
 +
padding-bottom:5px;
 +
padding-left:5px;
 +
border:3px solid #000000;
 +
border-radius:10px;
 +
-moz-border-radius:10px;
 +
}
 +
</style>
 +
</body>
</body>
</html>
</html>

Latest revision as of 01:28, 27 September 2012

Team:HKUST-Hong Kong - 2012.igem.org

PROTOCOL

<<< Return to Notebook

Regulation and Control System

  1. BBA_K733001

  2. BBA_K733002

  3. BBA_K733003

  4. BBA_K733004

Retrieved from "http://2012.igem.org/Team:HKUST-Hong_Kong/Notebook/Protocol"