Team:HKUST-Hong Kong/Notebook/Protocol
From 2012.igem.org
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.Upper_Logos#HKUST_Logo{ | .Upper_Logos#HKUST_Logo{ | ||
- | width: | + | width:270px; |
height:80px; | height:80px; | ||
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- | background-size: | + | background-size:270px 80px; |
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+ | |||
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- | |||
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<body> | <body> | ||
<div id="Navigation_top"> | <div id="Navigation_top"> | ||
- | < | + | <a href="https://2012.igem.org/Main_Page"><img id="iGEM_Logo" class="Upper_Logos" src="https://static.igem.org/mediawiki/2012/3/32/Igem-logo.png"></a> |
- | < | + | <a href="http://www.ust.hk"><img id="HKUST_Logo" class="Upper_Logos" src=""></a> |
+ | <a href="https://2012.igem.org/Team:HKUST-Hong_Kong"><img id="HKUST_iGEM_Logo" class="Upper_Logos" src="https://static.igem.org/mediawiki/2012/4/43/295197_446935765350523_472857901_n.jpg"></a> | ||
<div class="Navigation_Buttons" id="Team" align="center"><h3><p>TEAM</p></h3></div> | <div class="Navigation_Buttons" id="Team" align="center"><h3><p>TEAM</p></h3></div> | ||
<div class="Navigation_Buttons" id="Project" align="center"><h3><p>PROJECT</p></h3></div> | <div class="Navigation_Buttons" id="Project" align="center"><h3><p>PROJECT</p></h3></div> | ||
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<div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Members">Members</a></p></div> | <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Members">Members</a></p></div> | ||
<div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Advisors">Advisors</a></p></div> | <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Advisors">Advisors</a></p></div> | ||
- | |||
</div> | </div> | ||
<div class="Navigation_Content" id="Project_Content"> | <div class="Navigation_Content" id="Project_Content"> | ||
- | <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Project_Abstraction"> | + | <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Project_Abstraction">Abstract</a></p></div> |
- | <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Background_and_Motive" | + | <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Background_and_Motive">Motive</a></p></div> |
- | <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/ | + | <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Design_Overview">Design - Overview</a></p></div> |
- | + | <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Design_Module">Design - Module</a></p></div> | |
- | <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/ | + | <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Design_Chassis">Design - Chassis</a></p></div> |
- | + | ||
- | <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/ | + | |
- | + | ||
</div> | </div> | ||
<div class="Navigation_Content" id="Wet_lab_Content" align="center"> | <div class="Navigation_Content" id="Wet_lab_Content" align="center"> | ||
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<div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Characterization">Characterization</a></p></div> | <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Characterization">Characterization</a></p></div> | ||
<div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Achievement">Achievement</a></p></div> | <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Achievement">Achievement</a></p></div> | ||
- | <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/ | + | <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Future_Work">Future Work</a></p></div> |
</div> | </div> | ||
<div class="Navigation_Content" id="Human_practice_Content" align="center"> | <div class="Navigation_Content" id="Human_practice_Content" align="center"> | ||
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</div> | </div> | ||
<div class="Navigation_Content" id="Extras_Content" align="center"> | <div class="Navigation_Content" id="Extras_Content" align="center"> | ||
+ | <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Medal_Requirements">Medal Requirements</a></p></div> | ||
+ | <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Safety">Safety</a></p></div> | ||
+ | <div class="Content_Buttons""><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Attribution">Attribution</a></p></div> | ||
+ | <div class="Content_Buttons""><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Acknowledgement">Acknowledgement</a></p></div> | ||
<div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Glossary">Glossary</a></p></div> | <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Glossary">Glossary</a></p></div> | ||
- | |||
- | |||
</div> | </div> | ||
</div> | </div> | ||
<div><p align="center"><font size="20">PROTOCOL</font></p></div> | <div><p align="center"><font size="20">PROTOCOL</font></p></div> | ||
+ | <div><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Notebook"><<< Return to Notebook</a></p></div> | ||
+ | <div id="paragraph0" class="bodyParagraphs"> | ||
+ | <div align="left"> | ||
+ | <h1>General Protocol</h1> | ||
+ | </div><ol> | ||
+ | <li><p><a href="https://static.igem.org/mediawiki/2012/a/a4/Iodine_test_protocol.pdf" target="_blank">Amylase Test for Integration of <i>Bacillus subtilis</i></a></p></li> | ||
+ | <li><p><a href="https://static.igem.org/mediawiki/2012/6/6a/Transformation_of_Bacillus_subtilus.pdf" target="_blank">Transformation of Integration Vector into <i>Bacillus subtilis</i></a></p></li></ol> | ||
+ | </div> | ||
+ | |||
<div id="paragraph1" class="bodyParagraphs"> | <div id="paragraph1" class="bodyParagraphs"> | ||
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<h1>Target Binding</h1> | <h1>Target Binding</h1> | ||
</div> | </div> | ||
+ | <ol> | ||
+ | <li><p><a href="https://static.igem.org/mediawiki/2012/e/e7/HKUST_K733007_Specific_Protocol.pdf" target="_blank">BBa_K733007 construct protocol</a></p></li> | ||
+ | <li><p><a href="https://static.igem.org/mediawiki/2012/7/7d/K733008_Specific_Protocol.pdf" target="_blank">BBa_K733008 construct protocol</a></p></li></ol> | ||
<p></p> | <p></p> | ||
</div> | </div> | ||
+ | |||
+ | |||
<div id="paragraph2" class="bodyParagraphs"> | <div id="paragraph2" class="bodyParagraphs"> | ||
<div align="left"> | <div align="left"> | ||
- | <h1>Anti-tumor Molecule | + | <h1>Anti-tumor Molecule Secretion</h1> |
</div> | </div> | ||
- | <p> | + | <p><ol> |
- | <a href="https://static.igem.org/mediawiki/2012/7/71/BBa_K733005_construct_protocol.pdf">BBa_K733005 construct protocol</a><br> | + | <li><a href="https://static.igem.org/mediawiki/2012/7/71/BBa_K733005_construct_protocol.pdf" target="_blank">BBa_K733005 construct protocol</a></li><br> |
- | <a href="https://static.igem.org/mediawiki/2012/d/d4/BBa_K733006_construct_protocol.pdf">BBa_K733006 construct protocol</a><br> | + | <li><a href="https://static.igem.org/mediawiki/2012/d/d4/BBa_K733006_construct_protocol.pdf" target="_blank">BBa_K733006 construct protocol</a></li><br> |
- | <a href="">BBa_K733013 construct protocol</a><br> | + | <li><a href="https://static.igem.org/mediawiki/2012/1/16/BBa_K733013_construct_protocol.pdf" target="_blank">BBa_K733013 construct protocol</a></li><br> |
- | <a href="">BBa_K733014+ BBa_K733016 construct protocol</a><br> | + | <li><a href="https://static.igem.org/mediawiki/2012/c/c5/BBa_K733014%2B_BBa_K733016_construct_protocol.pdf" target="_blank">BBa_K733014+ BBa_K733016 construct protocol</a></li><br> |
- | <a href="">BBa_K733015+ BBa_K733017 construct protocol</a><br> | + | <li><a href="https://static.igem.org/mediawiki/2012/a/a7/BBa_K733015%2B_BBa_K733017_construct_protocol.pdf" target="_blank">BBa_K733015+ BBa_K733017 construct protocol</a></li><br> |
- | <a href="">BBa_K733016 transform in B.subtilis protocol</a><br> | + | <li><a href="https://static.igem.org/mediawiki/2012/8/8a/BBa_K733016_transform_in_B.subtilis_protocol.pdf" target="_blank">BBa_K733016 transform in <i>B.subtilis protocol</i></a></li><br> |
- | <a href="">BBa_K733017 transform in B.subtilis protocol</a><br> | + | <li><a href="https://static.igem.org/mediawiki/2012/1/1e/BBa_K733017_transform_in_B.subtilis_protocol.pdf" target="_blank">BBa_K733017 transform in <i>B.subtilis</i> protocol</a></li><br> |
- | </p> | + | </ol></p> |
</div> | </div> | ||
<div id="paragraph3" class="bodyParagraphs"> | <div id="paragraph3" class="bodyParagraphs"> | ||
<div align="left"> | <div align="left"> | ||
<h1>Regulation and Control System</h1> | <h1>Regulation and Control System</h1> | ||
- | </div> | + | </div><ol> |
- | + | <li><p><a href="https://static.igem.org/mediawiki/2012/6/6e/Protocol_for_the_construct_of_Ptms_in_pSB1C3.pdf" target="_blank">BBA_K733001</a></p></li> | |
- | </ | + | <li><p><a href="https://static.igem.org/mediawiki/2012/7/7e/Protocol_for_the_construct_of_xylR%2BPxylA_in_pSB1C3.pdf" target="_blank">BBA_K733002</a></p></li> |
- | < | + | <li><p><a href="https://static.igem.org/mediawiki/2012/6/6b/Protocol_for_the_construct_of_RBS%2BydcD_in_pSB1C3.pdf" target="_blank">BBA_K733003</a></p></li> |
+ | <li><p><a href="https://static.igem.org/mediawiki/2012/c/ca/Protocol_for_the_construct_of_RBS%2BydcE_in_pSB1C3.pdf" target="_blank">BBA_K733004</a></p></li> | ||
+ | |||
+ | </ol></div> | ||
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#paragraph1{ | #paragraph1{ | ||
background-color:#EDFFFF; | background-color:#EDFFFF; | ||
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} | } | ||
</style> | </style> | ||
+ | |||
+ | |||
+ | <div id="Sitemap"> | ||
+ | <div id="Sitemap_Home" align="center"> | ||
+ | <p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong"><b>Home</b></a></p> | ||
+ | </div> | ||
+ | <div class="Sitemap_Content"> | ||
+ | <li><p><b>Team</b><p><ol> | ||
+ | <li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Introduction">Introduction</a></p></li> | ||
+ | <li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Supervisor">Supervisor</a></p></li> | ||
+ | <li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Instructor">Instructor</a></p></li> | ||
+ | <li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Members">Members</a></p></li> | ||
+ | <li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Advisors">Advisors</a></p></li> | ||
+ | </ol> | ||
+ | </div> | ||
+ | <div class="Sitemap_Content"> | ||
+ | <li><p><b>Project</b></p><ol> | ||
+ | <li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Project_Abstraction">Abstract</a></p></li> | ||
+ | <li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Background_and_Motive">Motive</a></p></li> | ||
+ | <li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Design_Overview">Design - Overview</a></p></li> | ||
+ | <li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Design_Module">Design - Module</a></p></li> | ||
+ | <p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Module/Target_binding">Target Binding Module</a></p> | ||
+ | <p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Module/Anti_tumor">Anti-tumor Molecule Secretion Module</a></p> | ||
+ | <p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Module/Regulation_and_control">Regulation and Control Module</a></p> | ||
+ | <li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Design_Chassis">Design - Chassis</a></p></li></ol> | ||
+ | </div> | ||
+ | <div class="Sitemap_Content"> | ||
+ | <li><p><b>Wet Lab</b></p><ol> | ||
+ | <li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Parts_and_Device">Parts and Devices</a></p></li> | ||
+ | <p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Parts_and_Device">Overview</a></p> | ||
+ | <p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Construction">Construction</a></p> | ||
+ | <p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Assembly">Assembly</a></p> | ||
+ | <li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Notebook">Notebook</a></p></li> | ||
+ | <p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Notebook/Logbook">Logbook</a></p> | ||
+ | <p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Notebook/Protocol">Protocol</a></p> | ||
+ | <li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Characterization">Characterization</a></p></li> | ||
+ | <li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Achievement">Achievement</a></p></li> | ||
+ | <li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Future_Work">Future Work</a></p></li></ol> | ||
+ | </div> | ||
+ | <div class="Sitemap_Content"> | ||
+ | <li><p><b>Human Practice</b></p><ol> | ||
+ | <li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Overview">Overview</a></p></li> | ||
+ | <li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Interview">Interview</a></p></li> | ||
+ | <li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Presentation">Presentation</a></p></li> | ||
+ | <li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Calendar">Calendar</a></p></li></ol> | ||
+ | </div> | ||
+ | <div class="Sitemap_Content"> | ||
+ | <li><p><b>Extras</b></p><ol> | ||
+ | <li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Medal_Requirements">Medal Requirements</a></p></li> | ||
+ | <li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Safety">Safety</a></p></li> | ||
+ | <li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Attribution">Attribution</a></p></li> | ||
+ | <li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Acknowledgement">Acknowledgement</a></p></li> | ||
+ | <li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Glossary">Glossary</a></p></li></ol> | ||
+ | </div> | ||
+ | </div> | ||
+ | <style type="text/css"> | ||
+ | #Sitemap{ | ||
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Latest revision as of 01:28, 27 September 2012
PROTOCOL
General Protocol
Target Binding
Anti-tumor Molecule Secretion
Regulation and Control System
Project
Wet Lab
Human Practice