"
Team:UC Chile/Cyano/Labook/june
From 2012.igem.org
(Difference between revisions)
| (2 intermediate revisions not shown) | |||
| Line 30: | Line 30: | ||
<div id="navbar3"> | <div id="navbar3"> | ||
<ul>Lab book:<br> | <ul>Lab book:<br> | ||
| - | <li><a href="https://2012.igem.org/Team:UC_Chile/Labook/march">March</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/april">April</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/may">May</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/june">June</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/july">July</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/august">August</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/september">September</a></li> | + | <li><a href="https://2012.igem.org/Team:UC_Chile/Labook/march">March</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/april">April</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/may">May</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/june">June</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/july">July</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/august">August</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/september">September</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/october">October</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
| Line 37: | Line 37: | ||
<p><font size= "5">June</font></p> | <p><font size= "5">June</font></p> | ||
| + | |||
<font size="4">Monthly Summary</font> | <font size="4">Monthly Summary</font> | ||
| + | |||
We are waiting for Gibson enzymes to arrive. So there is no much wet lab work this month. | We are waiting for Gibson enzymes to arrive. So there is no much wet lab work this month. | ||
| + | |||
<font size="4">PCR runs</font> | <font size="4">PCR runs</font> | ||
| + | |||
While waiting for enzymes, all parts for future assemblies are being prepared. | While waiting for enzymes, all parts for future assemblies are being prepared. | ||
| Line 57: | Line 61: | ||
Gibson enzymes arrived late June. | Gibson enzymes arrived late June. | ||
| - | |||
| - | |||
| - | |||
| - | |||
{{UC_Chilefooter}} | {{UC_Chilefooter}} | ||
Latest revision as of 19:15, 26 October 2012
June
Monthly Summary
We are waiting for Gibson enzymes to arrive. So there is no much wet lab work this month.
PCR runs
While waiting for enzymes, all parts for future assemblies are being prepared.
Problematic parts: Kanr, P1003, B0014.
Tried with different DMSO concentrations. Best result at 5%.
Comments: we realized that our current primers work for P1003+B0015. Right primers were now ordered.
After arrival, no amplification for Kanr. Tried once more with an old Kanr purification: success.
New batch of competent cells prepared: Competence: 3.5x10^7 (not quite sure)
Gibson enzymes arrived late June.
