Team:UC Chile2/Cyanolux/Biobricks

From 2012.igem.org

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<h1>Constructs detail</h1>
<h1>Constructs detail</h1>
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[[File:UC_Chile-C1.1.jpg|center]]
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<html><img src="https://static.igem.org/mediawiki/2012/d/d0/UC_Chile-C1.1.jpg" align="right" width="800"><p style="width : 200px;">
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Integrative plasmid designed for the expression of RFP under synechocystis psbAB promoter also with a kanamycin resistance cassette for selection in cyanobacteria. The construct is flanked by neutral recombination sites RS1 and RS2, homologous to synechocystis chromosome.</p>
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Integrative plasmid designed for the expression of RFP under synechocystis psbAB promoter also with a kanamycin resistance cassette for selection in cyanobacteria. The construct is flanked by neutral recombination sites RS1 and RS2, homologous to synechocystis chromosome.</p></html>
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[[File:UC_Chile-C1.2.jpg|center]]
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<p>C1.2
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<html><img src="https://static.igem.org/mediawiki/2012/b/b9/UC_Chile-C1.2.jpg" align="right" width="800"><p style="width : 200px;">
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C1.2
This integrative plasmid is designed for the expression of RFP under synechocystis psaA2 promoter with a kanamycin resistance cassette for selection in cyanobacteria. The construct is flanked by neutral recombination sites RS1 and RS2, homologous to synechocystis chromosome.
This integrative plasmid is designed for the expression of RFP under synechocystis psaA2 promoter with a kanamycin resistance cassette for selection in cyanobacteria. The construct is flanked by neutral recombination sites RS1 and RS2, homologous to synechocystis chromosome.
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</p>
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</p></html>
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[[File:UC_Chile-C2.1.jpg|center]]
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<p>C2.1
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Construct designed from pPMQAK1 expression plasmid, which replicates both in E. coli and cyanobacteria and is biobrick-compatible. It codes for RFP under synechocystis psbAB promoter.</p>
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[[File:UC_Chile-C2.2.jpg|center]]
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<p>C2.2
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Construct designed from pPMQAK1 expression plasmid, which replicates both in E. coli and cyanobacteria and is biobrick-compatible. It codes for RFP under synechocystis psaA2 promoter.</p>
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[[File:UC_Chile-C3.1.jpg|center]]
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<p>C3
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Construct designed from pPMQAK1 expression plasmid, which replicates both in E. coli and cyanobacteria and is biobrick-compatible. It codes for RFP under synechocystis psbAB promoter and cuts out the whole Ccdb toxin gene.</p>
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[[File:UC_Chile-C3.2.jpg|center]]
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<html><img src="https://static.igem.org/mediawiki/2012/4/48/UC_Chile-C2.1.jpg" align="right" width="800"><p style="width : 200px;">
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C2.1
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Construct designed from pPMQAK1 expression plasmid, which replicates both in E. coli and cyanobacteria and is biobrick-compatible. It codes for RFP under synechocystis psbAB promoter.</p></html>
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<html><img src="https://static.igem.org/mediawiki/2012/c/c6/UC_Chile-C2.2.jpg" align="right" width="800"><p style="width : 200px;">
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C2.2
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Construct designed from pPMQAK1 expression plasmid, which replicates both in E. coli and cyanobacteria and is biobrick-compatible. It codes for RFP under synechocystis psaA2 promoter.</p></html>
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<html><img src="https://2012.igem.org/File:UC_Chile-C3.1.jpg" align="right" width="800"><p style="width : 200px;">
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C3
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Construct designed from pPMQAK1 expression plasmid, which replicates both in E. coli and cyanobacteria and is biobrick-compatible. It codes for RFP under synechocystis psbAB promoter and cuts out the whole Ccdb toxin gene.</p></html>
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<html><img src="https://2012.igem.org/File:UC_Chile-C3.2.jpg" align="right" width="800"><p style="width : 200px;"></p></html>
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<html><img src="https://2012.igem.org/File:C4.jpg" align="right" width="800"><p style="width : 200px;">Expression plasmid for the expression of LuxA and LuxB genes under psbAB promoter.
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<html><img src="https://2012.igem.org/File:C5.jpg" align="right" width="800"><p style="width : 200px;">C5<br>
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Integrative plasmid designed to integrate LuxA and LuxB and a kanamycin resistance cassette downstream the 3' end of the transaldolase gene whose mRNA levels where seen to oscillate in a circadian manner peaking at hour 14, just after dusk. It is expect the same for LuxAB mRNA levels.
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</p></html>
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[[File:c4.jpg]]
 
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<p>C4<br>
 
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Expression plasmid for the expression of LuxA and LuxB genes under psbAB promoter.
 
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[[File:c5.jpg]]
 
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<p>C5<br>
 
Integrative plasmid designed to integrate LuxA and LuxB and a kanamycin resistance cassette downstream the 3' end of the transaldolase gene whose mRNA levels where seen to oscillate in a circadian manner peaking at hour 14, just after dusk. It is expect the same for LuxAB mRNA levels.</p>
Integrative plasmid designed to integrate LuxA and LuxB and a kanamycin resistance cassette downstream the 3' end of the transaldolase gene whose mRNA levels where seen to oscillate in a circadian manner peaking at hour 14, just after dusk. It is expect the same for LuxAB mRNA levels.</p>
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[[File:UC_Chile-PSB1C3_INTK.jpg|center]]
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[[File:UC_Chile-PS81C3_INTS.jpg|center]]
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== Brief description of characterized biobricks utilized ==
== Brief description of characterized biobricks utilized ==

Latest revision as of 00:15, 25 September 2012

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012