Talk:Team:Columbia-Cooper-NYC/trial

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<a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Team">Biographies</a>
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<a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Facilitators">Facilitators</a>
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                <a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Data_and_Conclusions">Data and Conclusions</a>
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                <a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Future_Directions">Future Directions</a>
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                <a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/References">References</a>
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<a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Columbia_notebook_2">Genetics</a>
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<a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Columbia_notebook_1">Copper Etching</a>
 +
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        <li><a href="#" onmouseover="mopen('m6')" onmouseout="mclosetime()">Human Practice</a>
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<a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Collaborations">Collaboration</a>
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        <li><a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Safety" onmouseover="mopen('m7')" onmouseout="mclosetime()">Safety</a>
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                <a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Safety">Safety</a>
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                <a href="https://2012.igem.org/Security">Security</a>
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        <li><a href="#" onmouseover="mopen('m8')" onmouseout="mclosetime()">Sponsors</a>
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                <a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Sponsor_Us">Sponsor Us</a>
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<a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Acknowledgements">Acknowledgements</a>
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        <li><a href="https://2012.igem.org/Main_Page" onmouseover="mopen('m9')" onmouseout="mclosetime()">iGEM</a>
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<li><a class="head" href="#"> Introduction</a>
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<li><a class="head" href="#">Project Firefly</a>
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<li><a href="/Team:Cambridge/Bioluminescence/Luciferin_Regeneration">Luciferin recovery</a></li>
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<div style="width:730px; background-color:#5C3317;" class="secheader">The Project</div>
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<li><a href="/Team:Cambridge/Bioluminescence/Bacterial_Luciferases">Introduction</a></li>
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<li><a href="/Team:Cambridge/Bioluminescence/G28">The LuxBrick</a></li>
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<li><a href="/Team:Cambridge/Bioluminescence/Bacterial_Codon_optimisation">Codon optimisation</a></li>
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<li><a href="/Team:Cambridge/Bioluminescence/Vibrio_Modelling">Modelling</a></li>
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<li><a href="/Team:Cambridge/Tools/Ligate">Ligation Calculator</a></li>
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<ul class="llmenu llmenublue">
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The Columbia-Cooper iGEM team is working with Acidithiobacillus ferrooxidans to create a light-controlled printed circuit board manufacturing process. This bacteria’s metabolism relies on its ability to oxidize iron; the iron can then be used to oxidize, and in turn solubilize, copper. By genetically altering the bacteria, we intend to install a light sensitive mechanism which will enable us to etch copper in a desired pattern, leaving a finished circuit board. Once a blank printed circuit board is placed in a thin layer of solid media, the bacteria will be applied onto the surface of the media and light will be focused on it in a desired pattern. The light sensitive mechanism in ferrooxidans would activate and self-destruct in the pathway of the light. In the end, the circuit board will be "etched" by the bacteria everywhere but the illuminated spots, leaving the desired pattern behind on the circuit board. </p>
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<li><a href="/Team:Cambridge/Gibson/Introduction">Introduction</a></li>
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<li><a href="/Team:Cambridge/Gibson/Protocol">Protocol</a></li>
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<li><a class="head" href="#">Considerations</a>
 
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 +
<div id="leftbox" style="float:left; height:200px;  width:242px;" class="tophome">
 +
<div id="lefttop" style="background:#B87333; background-image:url('https://static.igem.org/mediawiki/2010/a/ad/Cam-Gradient-Overlay.png');" class="secheader">iGEM</div>
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<div id="leftcontent" style="background:-webkit-gradient(linear, left top, left bottom, from(#f2f2f2), to(#f8f8f8));
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background: -moz-linear-gradient(top, #f2f2f2, #f8f8f8); color:#333333; height:150px;">
 +
<p style="line-height:140%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;"> The <a style="color:#6bbe00 !important" href="https://igem.org/Main_Page">International Genetically Engineered Machine competition (iGEM)</a> is the premiere undergraduate Synthetic Biology competition. Student teams are given a kit of biological parts at the beginning of the summer from the <a style="color:#6bbe00 !important" href="http://partsregistry.org/Main_Page">Registry of Standard Biological Parts</a>. Working at their own schools over the summer, they use these parts and new parts of their own design to build biological systems and operate them in living cells. This project design and competition format is an exceptionally motivating and effective teaching method. In 2011 iGEM expanded to include a High School Division and an Entrepreneurship Division is 2012.</p>
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<div id="cbox" style="float:left; height:200px;  width:241px; margin-left:5px;" class="tophome">
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<div id="ctop" style="background:#238E23;background-image:url('https://static.igem.org/mediawiki/2010/a/ad/Cam-Gradient-Overlay.png')" class="secheader">Genetics</div>
 +
<div id="ccontent" style="background:-webkit-gradient(linear, left top, left bottom, from(#f2f2f2), to(#f8f8f8));
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 +
<p style="line-height:140%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;">About our genetics work.</a>.
 +
 +
<script>
 +
var dontclose=false;
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 +
var newDiv = document.createElement('div');
 +
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 +
newDiv.setAttribute('onclick',";hideOverlay();");
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 +
$('body')[0].appendChild(newDiv);
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 +
$('body')[0].removeChild($('#GibsonVidOverlay')[0]);
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<div id="rightbox" style="float:left;  height:200px; margin-left:5px; width:242px;" class="tophome">
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<div id="rtop" style="background:#B87333;background-image:url('https://static.igem.org/mediawiki/2010/a/ad/Cam-Gradient-Overlay.png')" class="secheader">Copper Etching</div>
 +
<div id="ccontent" style="background:-webkit-gradient(linear, left top, left bottom, from(#f2f2f2), to(#f8f8f8));
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background: -moz-linear-gradient(top, #f2f2f2, #f8f8f8); color:#333333; height:150px;">
 +
<p style="line-height:140%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;">About our copper work.</a>.
 +
</a>
 +
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 +
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<li><a class="head" href="/Team:Cambridge/Notebook/Week1">Notebook</a>
 
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<li><a href="/Team:Cambridge/Notebook/Week1">Week 1</a></li>
 
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<li><a href="/Team:Cambridge/Notebook/Week2">Week 2</a></li>
 
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<li><a href="/Team:Cambridge/Notebook/Week3">Week 3</a></li>
 
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<div id="leftbox" style="float:left; height:200px;  width:242px;" class="tophome">
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<div id="lefttop" style="background:#238E23; background-image:url('https://static.igem.org/mediawiki/2010/a/ad/Cam-Gradient-Overlay.png');" class="secheader">Biobrick</div>
<div id="leftcontent" style="background:-webkit-gradient(linear, left top, left bottom, from(#f2f2f2), to(#f8f8f8));  
<div id="leftcontent" style="background:-webkit-gradient(linear, left top, left bottom, from(#f2f2f2), to(#f8f8f8));  
background: -moz-linear-gradient(top, #f2f2f2, #f8f8f8); color:#333333; height:150px;">
background: -moz-linear-gradient(top, #f2f2f2, #f8f8f8); color:#333333; height:150px;">
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<p style="line-height:140%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;"> We placed genes from <a style="color:#6bbe00 !important" href="https://2010.igem.org/Team:Cambridge/Bioluminescence">fireflies</a> and bioluminescent <a style="color:#6bbe00 !important"  href="https://2010.igem.org/Team:Cambridge/Bioluminescence/Bacterial_Luciferases">bacteria</a> into E.coli. Codon optimisation and single amino acid mutagenesis allowed us to generate bright light output in a range of different colours. Future applications include  and quantitative <a style="color:#6bbe00 !important" href="https://2010.igem.org/Team:Cambridge/Tools/microMeasure">biosensors</a> and biological <a style="color:#6bbe00 !important" href="https://2010.igem.org/Team:Cambridge/Tools/Lighting">alternatives</a> to conventional lighting.</p>
+
<p style="line-height:140%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;"> This is how you link things <a style="color:#6bbe00 !important" href="https://2010.igem.org/Team:Cambridge/Bioluminescence">link</a> blahahahahjhmvgmh.</p>
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<div id="ctop" style="background:#fad72a;background-image:url('https://static.igem.org/mediawiki/2010/a/ad/Cam-Gradient-Overlay.png')" class="secheader">Multimedia</div>
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<div id="ctop" style="background:#B87333;background-image:url('https://static.igem.org/mediawiki/2010/a/ad/Cam-Gradient-Overlay.png')" class="secheader">Outreach</div>
<div id="ccontent" style="background:-webkit-gradient(linear, left top, left bottom, from(#f2f2f2), to(#f8f8f8));  
<div id="ccontent" style="background:-webkit-gradient(linear, left top, left bottom, from(#f2f2f2), to(#f8f8f8));  
background: -moz-linear-gradient(top, #f2f2f2, #f8f8f8); color:#333333; height:150px;">
background: -moz-linear-gradient(top, #f2f2f2, #f8f8f8); color:#333333; height:150px;">
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<p style="line-height:140%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;">If you want a break from hard-core science, check out our Gibson Assembly  <a  style="color:#d9b400 !important" onclick="displayGibson('http://www.youtube.com/v/WCWjJFU1be8?fs=1&amp;hl=en_GB'); return false;" href="http://www.youtube.com/watch?v=WCWjJFU1be8">music video</a>.<br /><br />You can also see view videos of our <a style="color:#d9b400 !important" onclick="displayGibson('http://www.youtube.com/v/tUFscEVK5Ks?fs=1&amp;hl=en_GB'); return false;" href="http://www.youtube.com/watch?v=WCWjJFU1be8">bacterial bubble lamp</a> and <a style="color:#d9b400 !important" onclick="displayGibson('http://www.cambridgeigem.org/video.php'); return false;" href="http://www.cambridgeigem.org/video.php?nojs=true">project overview</a>.
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<p style="line-height:140%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;">About our genetics work.</a>.
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<div id="rtop" style="background:#238E23;background-image:url('https://static.igem.org/mediawiki/2010/a/ad/Cam-Gradient-Overlay.png')" class="secheader">Sponsors</div>
<div id="rcontent" style="background:-webkit-gradient(linear, left top, left bottom, from(#f2f2f2), to(#f8f8f8));  
<div id="rcontent" style="background:-webkit-gradient(linear, left top, left bottom, from(#f2f2f2), to(#f8f8f8));  
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<div style="position:relative; top:10px;">
<div style="position:relative; top:10px;">
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<a href="http://www.columbia.edu/" id="leftbox" style="background:url('http://farm9.staticflickr.com/8314/8012579613_7f735be070_m.jpg'); position:absolute; width:120px; height:100px; left:0;top:0; background-repeat:no-repeat; display:block;text-align:center; padding-top:70px; background-position: center top; color:#e53500 !important;" >
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Gibson<br />Assembly
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Columbia<br />University
</a>
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<a href="http://cooper.edu/" id="rightbox" style="background:url('http://farm9.staticflickr.com/8318/8012585476_338eb1625f_m.jpg'); position:absolute; width:120px; height:100px; left:120px; text-align:center;top:0; background-repeat:no-repeat;display:block; padding-top:70px; background-position: center top; color:#e53500 !important;" />
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Ligation<br />Calculator
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Cooper<br />Union
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<div style="position:absolute; width:230px; left:5px; text-align:center;">A flask lit by E. coli transformed with one of our constructs </div>
 
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<div style="position:absolute; width:230px; left:240px; text-align:center;">E. coli transformed with our different coloured bioluminescent systems</div>
 
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<div style="position:absolute; width:220px; left:485px; text-align:center;">Team members illuminated only by our bacteria</div>
 
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</div>
 
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</p><p><br />
 
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Over the course of the summer we built a set of BioBricks to allow bioluminescence in a wide range of colours which have applications both as reporters for <a href="https://2010.igem.org/Team:Cambridge/Tools/microMeasure" class="external text" title="https://2010.igem.org/Team:Cambridge/Tools/microMeasure" rel="nofollow"><b>biosensors</b></a> and as <a href="https://2010.igem.org/Team:Cambridge/Tools/Lighting" class="external text" title="https://2010.igem.org/Team:Cambridge/Tools/Lighting" rel="nofollow"><b>natural light sources</b></a>. We also developed software tools to aid construction of BioBrick parts and devices.
 
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</p><p><h2 class="noline" style="margin-top:30px;">Project Firefly</h2>
 
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</p><p>We adopted a number of strategies to extend the use of <b>firefly luciferase</b>:
 
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</p>
 
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<ul><li> <a href="https://2010.igem.org/Team:Cambridge/Codons" class="external text" title="https://2010.igem.org/Team:Cambridge/Codons" rel="nofollow"><b>Codon optimisation</b></a> for increased light output
 
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</li><li> Use of a <a href="https://2010.igem.org/Team:Cambridge/Bioluminescence/Luciferin_Regeneration" class="external text" title="https://2010.igem.org/Team:Cambridge/Bioluminescence/Luciferin_Regeneration" rel="nofollow"><b>luciferin regenerating enzyme</b></a>.
 
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</li><li> Mutagenesis to create a number of <a href="https://2010.igem.org/Team:Cambridge/Bioluminescence/Colour" class="external text" title="https://2010.igem.org/Team:Cambridge/Bioluminescence/Colour" rel="nofollow"><b>different colours</b></a>
 
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</li></ul>
 
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<p><h2 class="noline" style="margin-top:30px;">Project Vibrio</h2>
 
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We complemented these firefly systems, which require the addition of the substrate luciferin, with light producing systems from <b>Vibrio fischeri</b>.  We believe we have created the <a href="https://2010.igem.org/Team:Cambridge/Bioluminescence/G28" class="external text" title="https://2010.igem.org/Team:Cambridge/Bioluminescence/G28" rel="nofollow"><b>first BioBrick</b></a> to emit light in normal E. coli strains without the addition of any external substrate.
 
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</p><p><h2 class="noline" style="margin-top:30px;">Tools</h2>
 
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</p><p>During our project we made extensive use of <a href="https://2010.igem.org/Team:Cambridge/Gibson/Introduction" class="external text" title="https://2010.igem.org/Team:Cambridge/Gibson/Introduction" rel="nofollow"><b>Gibson Assembly</b></a> to manufacture our parts, and have submitted an <a href="https://2010.igem.org/Team:Cambridge/Gibson/RFC" class="external text" title="https://2010.igem.org/Team:Cambridge/Gibson/RFC" rel="nofollow"><b>RFC</b></a> to the <a href="http://bbf.openwetware.org/" class="external text" title="http://bbf.openwetware.org/" rel="nofollow"><b>BioBricks Foundation</b></a> to help future teams make best use of this technique.
 
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</p><p>Along with this, we also constructed a number of tools to assist the synthetic biologists of the future:
 
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</p>
 
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<ul><li> <a href="https://2010.igem.org/Team:Cambridge/Tools/Gibson" class="external text" title="https://2010.igem.org/Team:Cambridge/Tools/Gibson" rel="nofollow"><b>Gibthon Construct Designer</b></a> allows the user to enter a series of BioBrick or GenBank IDs in a specific order and computes the appropriate primers for <a href="https://2010.igem.org/Team:Cambridge/Gibson/Introduction" class="external text" title="https://2010.igem.org/Team:Cambridge/Gibson/Introduction" rel="nofollow"><b>Gibson Assembly</b></a>.
 
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</li><li> <a href="https://2010.igem.org/Team:Cambridge/Tools/GenBank" class="external text" title="https://2010.igem.org/Team:Cambridge/Tools/GenBank" rel="nofollow"><b>BioBrick → GenBank</b></a> allows parts from the registry to be downloaded in .gb format, making them compatible with a wide range of biological software.
 
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</li><li> The <a href="https://2010.igem.org/Team:Cambridge/Tools/Ligate" class="external text" title="https://2010.igem.org/Team:Cambridge/Tools/Ligate" rel="nofollow"><b>Ligation Calculator</b></a> is a small calculator to help you work out the proportions to use for ligation in BioBrick assembly without having to worry about units.
 
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</li><li> The <a href="https://2010.igem.org/Team:Cambridge/Tools/Eglometer" class="external text" title="https://2010.igem.org/Team:Cambridge/Tools/Eglometer" rel="nofollow"><b>E.glometer</b></a> is a cheap, easily built, piece of electronics for measuring bioluminescence.  It allows scientists without access to expensive plate readers to measure bacterial light output and has potential applications in <a href="https://2010.igem.org/Team:Cambridge/Tools/microMeasure" class="external text" title="https://2010.igem.org/Team:Cambridge/Tools/microMeasure" rel="nofollow"><b>quantitative biosensors</b></a>.
 
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</li></ul>
 
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<p><h2 class="noline" style="margin-top:30px;">Achievements in iGEM competition</h2>
 
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</p>
 
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<ul><li> Finalist
 
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</li><li> Winner of "Best Wiki" award (awarded jointly to Cambridge and <a href="https://2010.igem.org/Team:Imperial_College_London" class="external text" title="https://2010.igem.org/Team:Imperial_College_London" rel="nofollow"><b>Imperial College London</b></a>)
 
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</li><li> Winner of the iGEMers Prize (awarded jointly to five iGEM teams)
 
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</li><li> Awarded a Gold Medal
 
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</li></ul>
 
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<p>Please see <a href="http://https://igem.org/Results" class="external text" title="http://https://igem.org/Results" rel="nofollow"><b>iGEM official results page</b></a> to see how all the teams did.
 
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</p><p><h2 class="noline" style="margin-top:30px;">In the news</h2>
 
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</p>
 
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<ul><li> <a href="http://www.newscientist.com/article/mg20827885.000-glowing-trees-could-light-up-city-streets.html" class="external text" title="http://www.newscientist.com/article/mg20827885.000-glowing-trees-could-light-up-city-streets.html" rel="nofollow">New Scientist article</a>
 
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</li><li> <a href="http://www.dailymail.co.uk/sciencetech/article-1333334/How-trees-glow-like-fireflies-day-replace-streetlights.html" class="external text" title="http://www.dailymail.co.uk/sciencetech/article-1333334/How-trees-glow-like-fireflies-day-replace-streetlights.html" rel="nofollow">Daily Mail article</a>
 
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</li><li> <a href="http://www.telegraph.co.uk/topics/christmas/8215302/The-science-of-Christmas-we-could-grow-our-own-fairy-lights-say-the-tree-wise-men.html" class="external text" title="http://www.telegraph.co.uk/topics/christmas/8215302/The-science-of-Christmas-we-could-grow-our-own-fairy-lights-say-the-tree-wise-men.html" rel="nofollow">The Telegraph Article</a>
 
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</li></ul>
 
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<div class="center"><div class="thumb tnone"><div class="thumbinner" style="width:702px;"><a href="/Image:Cambridge_team_pictwo2010.jpg" class="image" title="The team - in order - Anja Hohmann, Emily Knott, Hannah Copley, Will Handley, Theo Sanderson, Ben Reeve, Paul Masset, Peter Emmrich,  Bill Collins"><img alt="The team - in order - Anja Hohmann, Emily Knott, Hannah Copley, Will Handley, Theo Sanderson, Ben Reeve, Paul Masset, Peter Emmrich,  Bill Collins" src="/wiki/images/b/b0/Cambridge_team_pictwo2010.jpg" width="700" height="440" border="0" class="thumbimage" /></a>  <div class="thumbcaption">The team - in order - Anja Hohmann, Emily Knott, Hannah Copley, Will Handley, Theo Sanderson, Ben Reeve, Paul Masset, Peter Emmrich,  Bill Collins</div></div></div></div>
 
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Latest revision as of 14:50, 29 September 2012

Team:Cambridge

From 2010.igem.org



Biobrick

This is how you link things link blahahahahjhmvgmh.

Outreach

About our genetics work..

Caption
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Retrieved from "http://2012.igem.org/Talk:Team:Columbia-Cooper-NYC/trial"