Team:TU Darmstadt/Materials/tphC

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  rev: gtttcttc'''ctgcagcggccgctactagta'''ttagagcttgacgttggcggcc
  rev: gtttcttc'''ctgcagcggccgctactagta'''ttagagcttgacgttggcggcc
=== tphC Pst1 ===
=== tphC Pst1 ===
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[https://2012.igem.org/Team:TU_Darmstadt/Materials#Primers Primers] for removing a Pst1 site.
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[https://2012.igem.org/Team:TU_Darmstadt/Materials#Primers Primers] for removing a [https://2012.igem.org/Team:TU_Darmstadt/Materials/PstI Pst1] site.
       5'                            3'
       5'                            3'
  fwd: TACATCCCACCTGACGGCAGCCATGTTCATG
  fwd: TACATCCCACCTGACGGCAGCCATGTTCATG
  rev: CATGAACATGGCTGCCGTCAGGTGGGATGTA
  rev: CATGAACATGGCTGCCGTCAGGTGGGATGTA

Latest revision as of 20:49, 26 September 2012

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K808001 tphC]

Primers for the construction of a BioBrick containing the terephtalate periplasmatic binding protein of the tripartite tricarboxylate transporter family.

tphC BioBrick

     5' BioBrick                      Annealing 3'
fwd: gtttcttcgaattcgcggccgcttctagatgcgcaacgcatccattcgcag
rev: gtttcttcctgcagcggccgctactagtattagagcttgacgttggcggcc

tphC Pst1

Primers for removing a Pst1 site.

     5'                             3'
fwd: TACATCCCACCTGACGGCAGCCATGTTCATG
rev: CATGAACATGGCTGCCGTCAGGTGGGATGTA