Team:TU Darmstadt/Protocols

From 2012.igem.org

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(Cell culture)
 
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    <li><a href="/Team:TU_Darmstadt/Safety" title="Safety">Safety</a></li>
    <li><a href="/Team:TU_Darmstadt/Safety" title="Safety">Safety</a></li>
    <li><a href="/Team:TU_Darmstadt/Downloads" title="Downloads">Downloads</a></li></ul></li>
    <li><a href="/Team:TU_Darmstadt/Downloads" title="Downloads">Downloads</a></li></ul></li>
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<li><a href="/Team:TU_Darmstadt/Human_Practice" title="Human Practice">Human Practice</a><ul>
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<li><a href="/Team:TU_Darmstadt/Human_Practice" title="Human Practice">Human Practice</a></li>   
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    <li><a href="/Team:TU_Darmstadt/Human_Practice/Panel_Discussion " title="Panel Discussion">Panel Discussion</a></li>
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    <li><a href="/Team:TU_Darmstadt/Human_Practice/Symposia" title="Symposia">Symposia</a></li>
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    <li><a href="/Team:TU_Darmstadt/Human_Practice/Classes" title="Classes">Classes</a></li></ul></li>   
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<li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Sponsors</a><ul>
<li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Sponsors</a><ul>
    <li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Overview</a></li>
    <li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Overview</a></li>
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<span style="font-size:200%;"><span style="color:#00689D;">Protocols</span></span>
<span style="font-size:200%;"><span style="color:#00689D;">Protocols</span></span>
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The following page(s) gives an overview of the protocols and possible modifications that where used and proven working during the iGEM PET.erminators project 2012.
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The following page(s) gives an overview of the protocols and possible modifications that where used and proven working during the [https://2012.igem.org iGEM] PET.erminators [https://2012.igem.org/Team:TU_Darmstadt/Project project 2012]. The corresponding [https://2012.igem.org/Team:TU_Darmstadt/Materials materials] can be found in the [https://2012.igem.org/Team:TU_Darmstadt/Materials materials section].
== In vivo ==
== In vivo ==
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Electrocompetent_cells Electrocompetent cells]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Electrocompetent_cells Electrocompetent cells]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Electroporation Electroporation]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Electroporation Electroporation]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Engineering_BioBrick_vectors_from_BioBrick_parts/Colony_PCR Engineering BioBrick vectors from BioBrick parts/Colony PCR]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Glycerine_stock Glycerine Stocks]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/In-fusion_BioBrick_assembly In-fusion BioBrick assembly]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Heat_Shock_Transformation Heat Shock Transformation]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Miniprep_/_Fermentas_kit Miniprep / Fermentas kit]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Miniprep#Fermentas_kit Miniprep / Fermentas kit]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Miniprep_/_Promega_kit Miniprep / Promega kit]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Miniprep#Promega_kit Miniprep / Promega kit]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Miniprep_/_Qiagen_kit Miniprep / Qiagen kit]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Miniprep#Qiagen_kit Miniprep / Qiagen kit]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Transformation_of_chemically_competent_cells Transformation of chemically competent cells]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Protein_Expression#Protein_Expression_Est13 Protein Expression Est13]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Protein_Expression#Protein_Expression_FsC Protein Expression FsC]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Western_Blot Western blot]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Western_Blot Western blot]
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...3 Uhr ich geh ins Bett, mal wieder ...
 
=== [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Comamonas_testosteroni Comamonas testosteroni] ===
=== [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Comamonas_testosteroni Comamonas testosteroni] ===
* [http://www.dsmz.de/fileadmin/Bereiche/Microbiology/Dateien/Kultivierungshinweise/engl_Opening.pdf Reviving a freez dried cell culture]
* [http://www.dsmz.de/fileadmin/Bereiche/Microbiology/Dateien/Kultivierungshinweise/engl_Opening.pdf Reviving a freez dried cell culture]
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* ...
 
== In vitro ==
== In vitro ==
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Agarose_gel_electrophoresis Agarose gel electrophoresis]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Agarose_gel_electrophoresis Agarose gel electrophoresis]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/PCR_techniques PCR techniques]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/PCR_techniques PCR techniques]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/SOE-PCR SOE-PCR]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/PCR#SOE_PCR SOE-PCR]
 +
 
=== [http://en.wikipedia.org/wiki/DNA DNA] ===
=== [http://en.wikipedia.org/wiki/DNA DNA] ===
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Agarose_gel_electrophoresis Agarose gel electrophoresis]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Agarose_gel_electrophoresis Agarose gel electrophoresis]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Annealing_and_primer_extension Annealing and primer extension]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Ammonium_sulfate_precipitation Ammonium acetate precipitation]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Annealing_primers Annealing primers]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Assembly_PCR Assembly PCR]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Cloning Cloning]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Cloning Cloning]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Dephosphorylation Dephosphorylation]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/DNA_Ligation DNA Ligation]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/DNA_Ligation DNA Ligation]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/ DNA Ligation à la group transport]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/DNA_Quantification_/_NanoDrop DNA Quantification / NanoDrop]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/DNA_Quantification_/_NanoDrop DNA Quantification / NanoDrop]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/DNA_Synthesis_from_Oligos DNA Synthesis from Oligos]
 
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Engineering_BioBrick_vectors_from_BioBrick_parts/Colony_PCR Engineering BioBrick vectors from BioBrick parts/Colony PCR]
 
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Miniprep Miniprep]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Miniprep Miniprep]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/mutagenic_PCR Mutagenic PCR]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/PCR PCR]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/PCR PCR]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Polyacrylamide_gel_electrophoresis Polyacrylamide gel electrophoresis]
 
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Primer_Design Primer Design]
 
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Purification_of_DNA  Purification of DNA]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Purification_of_DNA  Purification of DNA]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Restriction_digest Restriction digest]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Restriction_digest Restriction digest]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Resuspension_of_primers Resuspension of primers]
 
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Salting_Out Salting Out]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Salting_Out Salting Out]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Sequencing_DNA Sequencing DNA]
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* [http://www.eurofinsdna.com/de/home.html?wmc=GA_DE-Corporate&gclid=CODAs8CZ1LICFQVf3god9AIAag Sequencing DNA]
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=== [http://en.wikipedia.org/wiki/RNA RNA] ===
 
=== [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Protein Protein] ===
=== [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Protein Protein] ===
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* [https://2012.igem.org/wiki/index.php?title=Team:TU_Darmstadt/Materials/SDS-Page SDS-Page]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Activity_assay Activity assay]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Affinity_chromatography Affinity chromatography]
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== In silico ==
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/IMAC Immobilized metal ion affinity chromatography (IMAC)]
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=== Data analysis ===
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/pNP_Assay#Bacteria pNP-Assay Bacteria]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Information_theory Information theory]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/pNP_Assay#Enzyme pNP-Assay Enzyme]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/R_Programming R Programming]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Proteinpurification Proteinpurification]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Mathlab_/_Simulink Mathlab / Simulink]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Purification_of_Periplasmatic_Proteins Purification of Periplasmatic Proteins]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/SDS-Page SDS-Page]
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=== Databases ===
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/PDB PDB]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/CAS CAS]
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=== Modelling ===
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* [https://2012.igem.org/Team:TU_Darmstadt/Labjournal/Simulation/GNM Gaussian Network Modelling]
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=== Sequence analysis ===
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/BLAST BLAST]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/SAGE SAGE]
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=== Structure analysis ===
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/PDB PDB]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Protein_structure_prediction Protein structure prediction]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/RNA_secondary_structure_prediction RNA secondary structure prediction]
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== Material Science ==
== Material Science ==
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Synthesis_of_paranitrophenylesters_with_acyl_chlorides_in_presence_of_triethylamine Synthesis of paranitrophenylesters with acyl chlorides in presence of triethylamine]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Synthesis_of_paranitrophenylesters_with_acyl_chlorides_in_presence_of_triethylamine Synthesis of paranitrophenylesters with acyl chlorides in presence of triethylamine]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Synthesis_of_polyethylene_terephthalate_in_presence_of_sulfuric_acid Synthesis of polyethylene terephthalate in presence of sulfuric acid]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Surface_analysis_of_polyethylene_terephthalate_with_atomic_force_microscopy Surface analysis of polyethylene terephthalate with atomic force microscopy]
== Miscellaneous ==
== Miscellaneous ==
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=== Cell culture ===
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=== Staining ===
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Pouring_LB_plates Pouring LB plates]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Antibody_staining Antibody staining]
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=== Microscopy ===
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=== Analytics ===
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Determining_an_objects_size Determining an objects size]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/TEM-EDX TEM-EDX Measurements]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Preparing_slides Preparing slides]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/GC-MS GC-MS Measurements]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Staining Staining]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/SamplePreparationEDX Preparation of Escherichia Coli for the energy dispersive X-ray spectroscopy (EDX)]
=== ILDs / Improvised Lab Devices ===
=== ILDs / Improvised Lab Devices ===

Latest revision as of 21:45, 26 September 2012

Protocols

The following page(s) gives an overview of the protocols and possible modifications that where used and proven working during the iGEM PET.erminators project 2012. The corresponding materials can be found in the materials section.

Contents

In vivo

[http://en.wikipedia.org/wiki/E._coli Escherichia coli]

Comamonas testosteroni

  • [http://www.dsmz.de/fileadmin/Bereiche/Microbiology/Dateien/Kultivierungshinweise/engl_Opening.pdf Reviving a freez dried cell culture]

In vitro

Nucleic acids

[http://en.wikipedia.org/wiki/DNA DNA]

Protein

Material Science

Miscellaneous

Staining

Analytics

ILDs / Improvised Lab Devices