"
Team:TU Darmstadt/Protocols
From 2012.igem.org
(Difference between revisions)
(→Cell culture) |
|||
| (64 intermediate revisions not shown) | |||
| Line 27: | Line 27: | ||
<li><a href="/Team:TU_Darmstadt/Safety" title="Safety">Safety</a></li> | <li><a href="/Team:TU_Darmstadt/Safety" title="Safety">Safety</a></li> | ||
<li><a href="/Team:TU_Darmstadt/Downloads" title="Downloads">Downloads</a></li></ul></li> | <li><a href="/Team:TU_Darmstadt/Downloads" title="Downloads">Downloads</a></li></ul></li> | ||
| - | <li><a href="/Team:TU_Darmstadt/Human_Practice" title="Human Practice">Human Practice</a | + | <li><a href="/Team:TU_Darmstadt/Human_Practice" title="Human Practice">Human Practice</a></li> |
| - | + | ||
| - | + | ||
| - | + | ||
<li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Sponsors</a><ul> | <li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Sponsors</a><ul> | ||
<li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Overview</a></li> | <li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Overview</a></li> | ||
| Line 42: | Line 39: | ||
<span style="font-size:200%;"><span style="color:#00689D;">Protocols</span></span> | <span style="font-size:200%;"><span style="color:#00689D;">Protocols</span></span> | ||
| - | The following page(s) gives an overview of the protocols and possible modifications that where used and proven working during the iGEM PET.erminators project 2012. | + | The following page(s) gives an overview of the protocols and possible modifications that where used and proven working during the [https://2012.igem.org iGEM] PET.erminators [https://2012.igem.org/Team:TU_Darmstadt/Project project 2012]. The corresponding [https://2012.igem.org/Team:TU_Darmstadt/Materials materials] can be found in the [https://2012.igem.org/Team:TU_Darmstadt/Materials materials section]. |
== In vivo == | == In vivo == | ||
| Line 53: | Line 50: | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Electrocompetent_cells Electrocompetent cells] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Electrocompetent_cells Electrocompetent cells] | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Electroporation Electroporation] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Electroporation Electroporation] | ||
| - | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/ | + | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Glycerine_stock Glycerine Stocks] |
| - | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/ | + | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Heat_Shock_Transformation Heat Shock Transformation] |
| - | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/ | + | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Miniprep#Fermentas_kit Miniprep / Fermentas kit] |
| - | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/ | + | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Miniprep#Promega_kit Miniprep / Promega kit] |
| - | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/ | + | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Miniprep#Qiagen_kit Miniprep / Qiagen kit] |
| - | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/ | + | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Protein_Expression#Protein_Expression_Est13 Protein Expression Est13] |
| + | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Protein_Expression#Protein_Expression_FsC Protein Expression FsC] | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Western_Blot Western blot] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Western_Blot Western blot] | ||
| - | |||
=== [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Comamonas_testosteroni Comamonas testosteroni] === | === [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Comamonas_testosteroni Comamonas testosteroni] === | ||
* [http://www.dsmz.de/fileadmin/Bereiche/Microbiology/Dateien/Kultivierungshinweise/engl_Opening.pdf Reviving a freez dried cell culture] | * [http://www.dsmz.de/fileadmin/Bereiche/Microbiology/Dateien/Kultivierungshinweise/engl_Opening.pdf Reviving a freez dried cell culture] | ||
| - | |||
== In vitro == | == In vitro == | ||
| Line 70: | Line 66: | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Agarose_gel_electrophoresis Agarose gel electrophoresis] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Agarose_gel_electrophoresis Agarose gel electrophoresis] | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/PCR_techniques PCR techniques] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/PCR_techniques PCR techniques] | ||
| - | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/ | + | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/PCR#SOE_PCR SOE-PCR] |
| + | |||
=== [http://en.wikipedia.org/wiki/DNA DNA] === | === [http://en.wikipedia.org/wiki/DNA DNA] === | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Agarose_gel_electrophoresis Agarose gel electrophoresis] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Agarose_gel_electrophoresis Agarose gel electrophoresis] | ||
| - | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/ | + | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Ammonium_sulfate_precipitation Ammonium acetate precipitation] |
| - | + | ||
| - | + | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Cloning Cloning] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Cloning Cloning] | ||
| + | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Dephosphorylation Dephosphorylation] | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/DNA_Ligation DNA Ligation] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/DNA_Ligation DNA Ligation] | ||
| + | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/ DNA Ligation à la group transport] | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/DNA_Quantification_/_NanoDrop DNA Quantification / NanoDrop] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/DNA_Quantification_/_NanoDrop DNA Quantification / NanoDrop] | ||
| - | |||
| - | |||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Miniprep Miniprep] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Miniprep Miniprep] | ||
| + | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/mutagenic_PCR Mutagenic PCR] | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/PCR PCR] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/PCR PCR] | ||
| - | |||
| - | |||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Purification_of_DNA Purification of DNA] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Purification_of_DNA Purification of DNA] | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Restriction_digest Restriction digest] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Restriction_digest Restriction digest] | ||
| - | |||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Salting_Out Salting Out] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Salting_Out Salting Out] | ||
| - | * [ | + | * [http://www.eurofinsdna.com/de/home.html?wmc=GA_DE-Corporate&gclid=CODAs8CZ1LICFQVf3god9AIAag Sequencing DNA] |
| - | |||
=== [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Protein Protein] === | === [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Protein Protein] === | ||
| - | + | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Activity_assay Activity assay] | |
| - | + | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Affinity_chromatography Affinity chromatography] | |
| - | + | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/IMAC Immobilized metal ion affinity chromatography (IMAC)] | |
| - | + | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/pNP_Assay#Bacteria pNP-Assay Bacteria] | |
| - | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/ | + | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/pNP_Assay#Enzyme pNP-Assay Enzyme] |
| - | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/ | + | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Proteinpurification Proteinpurification] |
| - | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/ | + | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Purification_of_Periplasmatic_Proteins Purification of Periplasmatic Proteins] |
| - | + | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/SDS-Page SDS-Page] | |
| - | + | ||
| - | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/ | + | |
| - | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/ | + | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/ | + | |
| - | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/ | + | |
| - | + | ||
| - | + | ||
| - | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/ | + | |
| - | + | ||
| - | + | ||
== Material Science == | == Material Science == | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Synthesis_of_paranitrophenylesters_with_acyl_chlorides_in_presence_of_triethylamine Synthesis of paranitrophenylesters with acyl chlorides in presence of triethylamine] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Synthesis_of_paranitrophenylesters_with_acyl_chlorides_in_presence_of_triethylamine Synthesis of paranitrophenylesters with acyl chlorides in presence of triethylamine] | ||
| - | + | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Synthesis_of_polyethylene_terephthalate_in_presence_of_sulfuric_acid Synthesis of polyethylene terephthalate in presence of sulfuric acid] | |
| + | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Surface_analysis_of_polyethylene_terephthalate_with_atomic_force_microscopy Surface analysis of polyethylene terephthalate with atomic force microscopy] | ||
== Miscellaneous == | == Miscellaneous == | ||
| - | === | + | === Staining === |
| - | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/ | + | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Antibody_staining Antibody staining] |
| - | === | + | === Analytics === |
| - | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/ | + | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/TEM-EDX TEM-EDX Measurements] |
| - | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/ | + | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/GC-MS GC-MS Measurements] |
| - | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/ | + | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/SamplePreparationEDX Preparation of Escherichia Coli for the energy dispersive X-ray spectroscopy (EDX)] |
=== ILDs / Improvised Lab Devices === | === ILDs / Improvised Lab Devices === | ||
Latest revision as of 21:45, 26 September 2012
Protocols
The following page(s) gives an overview of the protocols and possible modifications that where used and proven working during the iGEM PET.erminators project 2012. The corresponding materials can be found in the materials section.
Contents |
In vivo
[http://en.wikipedia.org/wiki/E._coli Escherichia coli]
- Bacterial cell culture
- Bacterial transformation aka TRAFO
- Cell counting/plating
- Chemically competent cells
- Colony PCR
- Electrocompetent cells
- Electroporation
- Glycerine Stocks
- Heat Shock Transformation
- Miniprep / Fermentas kit
- Miniprep / Promega kit
- Miniprep / Qiagen kit
- Protein Expression Est13
- Protein Expression FsC
- Western blot
Comamonas testosteroni
- [http://www.dsmz.de/fileadmin/Bereiche/Microbiology/Dateien/Kultivierungshinweise/engl_Opening.pdf Reviving a freez dried cell culture]
In vitro
Nucleic acids
[http://en.wikipedia.org/wiki/DNA DNA]
- Agarose gel electrophoresis
- Ammonium acetate precipitation
- Cloning
- Dephosphorylation
- DNA Ligation
- DNA Ligation à la group transport
- DNA Quantification / NanoDrop
- Miniprep
- Mutagenic PCR
- PCR
- Purification of DNA
- Restriction digest
- Salting Out
- [http://www.eurofinsdna.com/de/home.html?wmc=GA_DE-Corporate&gclid=CODAs8CZ1LICFQVf3god9AIAag Sequencing DNA]
Protein
- Activity assay
- Affinity chromatography
- Immobilized metal ion affinity chromatography (IMAC)
- pNP-Assay Bacteria
- pNP-Assay Enzyme
- Proteinpurification
- Purification of Periplasmatic Proteins
- SDS-Page
Material Science
- Synthesis of paranitrophenylesters with acyl chlorides in presence of triethylamine
- Synthesis of polyethylene terephthalate in presence of sulfuric acid
- Surface analysis of polyethylene terephthalate with atomic force microscopy
Miscellaneous
Staining
Analytics
- TEM-EDX Measurements
- GC-MS Measurements
- Preparation of Escherichia Coli for the energy dispersive X-ray spectroscopy (EDX)
ILDs / Improvised Lab Devices
- x bp Primer Designer
- Enhancing a thermo cyclers cooling capabilites
- Gel pocket size reduction
- Growing iGEMlers
