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Team:TU Darmstadt/Materials/PstI
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<li><a href="/Team:TU_Darmstadt/Safety" title="Safety">Safety</a></li> | <li><a href="/Team:TU_Darmstadt/Safety" title="Safety">Safety</a></li> | ||
<li><a href="/Team:TU_Darmstadt/Downloads" title="Downloads">Downloads</a></li></ul></li> | <li><a href="/Team:TU_Darmstadt/Downloads" title="Downloads">Downloads</a></li></ul></li> | ||
| - | <li><a href="/Team:TU_Darmstadt/Human_Practice" title="Human Practice">Human Practice</a | + | <li><a href="/Team:TU_Darmstadt/Human_Practice" title="Human Practice">Human Practice</a></li> |
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<li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Sponsors</a><ul> | <li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Sponsors</a><ul> | ||
<li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Overview</a></li> | <li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Overview</a></li> | ||
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== PstI == | == PstI == | ||
=== About === | === About === | ||
| - | PstI is a endonucleasic [https://2012.igem.org/Team:TU_Darmstadt/Materials#Restriction_enzymes restriction enzyme] isolated from ''[http://en.wikipedia.org/wiki/Providencia_stuartii Providencia stuartii 164]''. It creates stick ends with | + | PstI is a endonucleasic [https://2012.igem.org/Team:TU_Darmstadt/Materials#Restriction_enzymes restriction enzyme] isolated from ''[http://en.wikipedia.org/wiki/Providencia_stuartii Providencia stuartii 164]''. It creates stick ends with 3' end overhang. |
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=== Working conditions === | === Working conditions === | ||
The recognition motive of EcoRI is: | The recognition motive of EcoRI is: | ||
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3' GACGTC --> 3'---G ACGTC---5' | 3' GACGTC --> 3'---G ACGTC---5' | ||
'''Buffer:''' NEBuffer 3 + BSA | '''Buffer:''' NEBuffer 3 + BSA | ||
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PstI requires at least 1bp overhang on its cutting site (with reduced cleavage efficiency 37%), more than 3bp (98%) are recommended.<sup>[1]</sup> | PstI requires at least 1bp overhang on its cutting site (with reduced cleavage efficiency 37%), more than 3bp (98%) are recommended.<sup>[1]</sup> | ||
=== Supplier === | === Supplier === | ||
Latest revision as of 20:37, 26 September 2012
Contents |
PstI
About
PstI is a endonucleasic restriction enzyme isolated from [http://en.wikipedia.org/wiki/Providencia_stuartii Providencia stuartii 164]. It creates stick ends with 3' end overhang.
Working conditions
The recognition motive of EcoRI is:
5' CTGCAG --> 5'---CTGCA G---3' 3' GACGTC --> 3'---G ACGTC---5'
Buffer: NEBuffer 3 + BSA
PstI requires at least 1bp overhang on its cutting site (with reduced cleavage efficiency 37%), more than 3bp (98%) are recommended.[1]
Supplier
- NEB http://www.neb.com/nebecomm/products/productR3140.asp
References
[1] http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/cleavage_linearized_vector.asp
