Team:Arizona State

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Diarrheic pathogens including E.coli O157:H7 serotype, campylobacter, shigella, and salmonella often contaminate drinking water supplies in developing nations and are responsible for approximately 1.5 million worldwide annual deaths.   Current technologies for detection of bacteria include DNA hybridization FRET signaling, electrical detection via immobilized antimicrobial peptides, and PCR amplification followed by gel visualization. Our method of bacterial detection fills a niche in biosensor technology.  Our design implies lower costs, higher portability, and a more rapid signal output than most bacterial biosensors. Additionally, our interchangeable DNA probe confers modularity, allowing for a range of bacterial detection. Using a novel split beta-galactosidase complementation assay, we have designed three unique chimeric proteins that recognize and bind to specific pathogenic markers and create a functioning beta-galactosidase enzyme. This functioning enzyme unit then cleaves x-gal and produces a colorimetric output signal. Our research demonstrates success in initial stages of chimeric protein assembly.   
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    <td width="57"><img src="https://static.igem.org/mediawiki/2012/1/13/Raindrop.png" alt="" width="53" height="62" /></td>
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    <td width="415"><h4>The Problem:<br />
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      Childhood Diarrhea From Contaminated Water</h4></td>
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    <td colspan="2"><a class="linkopacity" href="https://2012.igem.org/Team:Arizona_State/Problem"><img src="https://static.igem.org/mediawiki/2012/0/03/Asuigem_diseasemap.png" alt="" width="450" /></td>
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    <td colspan="2"><medium>Viewed as a minor inconvenience in the developed world, diarrhea can be a death sentence in developing countries. Diarrhea can be life threatening as it causes severe dehydration as a result of extensive fluid loss. An estimated 2.0 billion cases of diarrhea occur each year amongst children under five years of age. Of these cases, 1.5 million children die. The major bacterial pathogens that most frequently cause acute childhood diarrhea are  <i>E. coli, Shigella, Campylobacter</i> and <i>Salmonella</i>. Currently, existing biosensors for water-borne pathogens are either costly, inaccessible to developing countries, require expensive equipment constrained to a lab setting, difficult to use without training, and not very reliable...<a href="https://2012.igem.org/Team:Arizona_State/Problem"><strong>More &gt;</strong></a></small></td>
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          <td width="155"><p align="center"><a class="linkopacity" href="https://2012.igem.org/Team:Arizona_State/Overview">
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          <td><p align="center"><a class="linkopacity" href="https://2012.igem.org/Team:Arizona_State/International">
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Latest revision as of 03:39, 27 October 2012



The Problem:
Childhood Diarrhea From Contaminated Water
Viewed as a minor inconvenience in the developed world, diarrhea can be a death sentence in developing countries. Diarrhea can be life threatening as it causes severe dehydration as a result of extensive fluid loss. An estimated 2.0 billion cases of diarrhea occur each year amongst children under five years of age. Of these cases, 1.5 million children die. The major bacterial pathogens that most frequently cause acute childhood diarrhea are E. coli, Shigella, Campylobacter and Salmonella. Currently, existing biosensors for water-borne pathogens are either costly, inaccessible to developing countries, require expensive equipment constrained to a lab setting, difficult to use without training, and not very reliable...More >

 

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