Team:KAIST Korea/Notebook Labnote/2012 9

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<div class="date">09/08/2012</div></br>
<div class="date">09/08/2012</div></br>
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<b>Induction of Moth_1197, Moth_1198 gene (sampling) and running SDS-PAGE gel. </b>
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<div id="note_title"><b>Induction of Moth_1197, Moth_1198 gene (sampling) and running SDS-PAGE gel. </b></div>
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We induced Moth-1197 and Moth_1198 gene in pTrcHis2A and pBAD vector with various IPTG and L-arabinose conditions: IPTG 0.5mM, 1mM and Arabinose 10mM, 30mM (0.02% and 0.2%). Temperature conditions are : 37℃, 30℃. Cells were cultured anaerobically in the anaerobic chamber. After 6 hours of induction, we run the sample into SDS-PAGE gel. The gel is stained with coomassie blue for 1 hour and de-stained for overnight.
We induced Moth-1197 and Moth_1198 gene in pTrcHis2A and pBAD vector with various IPTG and L-arabinose conditions: IPTG 0.5mM, 1mM and Arabinose 10mM, 30mM (0.02% and 0.2%). Temperature conditions are : 37℃, 30℃. Cells were cultured anaerobically in the anaerobic chamber. After 6 hours of induction, we run the sample into SDS-PAGE gel. The gel is stained with coomassie blue for 1 hour and de-stained for overnight.

Revision as of 18:12, 6 September 2012

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09/08/2012

Induction of Moth_1197, Moth_1198 gene (sampling) and running SDS-PAGE gel.


We induced Moth-1197 and Moth_1198 gene in pTrcHis2A and pBAD vector with various IPTG and L-arabinose conditions: IPTG 0.5mM, 1mM and Arabinose 10mM, 30mM (0.02% and 0.2%). Temperature conditions are : 37℃, 30℃. Cells were cultured anaerobically in the anaerobic chamber. After 6 hours of induction, we run the sample into SDS-PAGE gel. The gel is stained with coomassie blue for 1 hour and de-stained for overnight. Anaerobic chamber ?
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