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Team:UIUC-Illinois/Notebook/Protocols/GelElectrophoresis
From 2012.igem.org
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<h2>To check PCR results</h2><br/> | <h2>To check PCR results</h2><br/> | ||
| - | What you need:<br/> | + | What you need:<br/><br/> |
- High-melt agarose<br/> | - High-melt agarose<br/> | ||
- TAE buffer 1X<br/> | - TAE buffer 1X<br/> | ||
- Autoclaved container <br/><br/> | - Autoclaved container <br/><br/> | ||
| - | Procedure:<br/> | + | Procedure:<br/><br/> |
1. Add 3.2g high-melt agarose to 400 mL 1X TAE buffer (50 uL agarose gel, 250 mL 1X TAE buffer for the small gel box) <br/> | 1. Add 3.2g high-melt agarose to 400 mL 1X TAE buffer (50 uL agarose gel, 250 mL 1X TAE buffer for the small gel box) <br/> | ||
2. Microwave flask or bottle (cap placed loosely if bottle) of mixed solution to dissolve gel in 1 minute increments until all agarose is dissolved<br/> | 2. Microwave flask or bottle (cap placed loosely if bottle) of mixed solution to dissolve gel in 1 minute increments until all agarose is dissolved<br/> | ||
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<h2>To do a gel purification</h2><br/> | <h2>To do a gel purification</h2><br/> | ||
| - | What you need:<br/> | + | What you need:<br/><br/> |
- Low-melt agarose<br/> | - Low-melt agarose<br/> | ||
- TAE buffer 1X<br/> | - TAE buffer 1X<br/> | ||
- Autoclaved container <br/><br/> | - Autoclaved container <br/><br/> | ||
| - | Procedure:<br/> | + | Procedure:<br/><br/> |
1. Add 4.0g low-melt agarose to 400 mL 1X TAE buffer<br/> | 1. Add 4.0g low-melt agarose to 400 mL 1X TAE buffer<br/> | ||
2. Microwave flask or bottle (cap placed loosely if bottle) of mixed solution to dissolve gel in 1 minute increments until all agarose is dissolved<br/> | 2. Microwave flask or bottle (cap placed loosely if bottle) of mixed solution to dissolve gel in 1 minute increments until all agarose is dissolved<br/> | ||
Latest revision as of 15:32, 4 June 2012
