Team:UIUC-Illinois/Notebook/Protocols/GelElectrophoresis

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<center><h1>Making Gel Electrophoresis gel solutions</h1></center> <br/><br/>
<center><h1>Making Gel Electrophoresis gel solutions</h1></center> <br/><br/>
-
<b><u>To check PCR results</u></b><br/><br/>
+
<h2>To check PCR results</h2><br/>
-
What you need:<br/>
+
What you need:<br/><br/>
- High-melt agarose<br/>
- High-melt agarose<br/>
-
- TAE buffer 1X<br/><br/>
+
- TAE buffer 1X<br/>
 +
- Autoclaved container <br/><br/>
-
Procedure:<br/>
+
Procedure:<br/><br/>
1. Add 3.2g high-melt agarose to 400 mL 1X TAE buffer (50 uL agarose gel, 250 mL 1X TAE buffer for the small gel box) <br/>
1. Add 3.2g high-melt agarose to 400 mL 1X TAE buffer (50 uL agarose gel, 250 mL 1X TAE buffer for the small gel box) <br/>
-
2. Microwave flask or bottle (cap placed loosely if bottle) of mixed solution to dissolve gel in 1 minute increments until all agarose is dissolved
+
2. Microwave flask or bottle (cap placed loosely if bottle) of mixed solution to dissolve gel in 1 minute increments until all agarose is dissolved<br/>
3. BE CAREFUL AS LIQUID CAN BE SUPERHEATED<br/><br/>
3. BE CAREFUL AS LIQUID CAN BE SUPERHEATED<br/><br/>
-
<b><u>To do a gel purification</u></b><br/><br/>
+
<h2>To do a gel purification</h2><br/>
-
What you need:<br/>
+
What you need:<br/><br/>
- Low-melt agarose<br/>
- Low-melt agarose<br/>
-
- TAE buffer 1X<br/><br/>
+
- TAE buffer 1X<br/>
-
Procedure:<br/>
+
- Autoclaved container <br/><br/>
 +
Procedure:<br/><br/>
1. Add 4.0g low-melt agarose to 400 mL 1X TAE buffer<br/>
1. Add 4.0g low-melt agarose to 400 mL 1X TAE buffer<br/>
2. Microwave flask or bottle (cap placed loosely if bottle) of mixed solution to dissolve gel in 1 minute increments until all agarose is dissolved<br/>
2. Microwave flask or bottle (cap placed loosely if bottle) of mixed solution to dissolve gel in 1 minute increments until all agarose is dissolved<br/>

Latest revision as of 15:32, 4 June 2012

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Protocols

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Making Gel Electrophoresis gel solutions



To check PCR results


What you need:

- High-melt agarose
- TAE buffer 1X
- Autoclaved container

Procedure:

1. Add 3.2g high-melt agarose to 400 mL 1X TAE buffer (50 uL agarose gel, 250 mL 1X TAE buffer for the small gel box)
2. Microwave flask or bottle (cap placed loosely if bottle) of mixed solution to dissolve gel in 1 minute increments until all agarose is dissolved
3. BE CAREFUL AS LIQUID CAN BE SUPERHEATED

To do a gel purification


What you need:

- Low-melt agarose
- TAE buffer 1X
- Autoclaved container

Procedure:

1. Add 4.0g low-melt agarose to 400 mL 1X TAE buffer
2. Microwave flask or bottle (cap placed loosely if bottle) of mixed solution to dissolve gel in 1 minute increments until all agarose is dissolved
3. BE CAREFUL AS LIQUID CAN BE SUPERHEATED

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