Team:UIUC-Illinois/Notebook/Protocols/Subculturing
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(Difference between revisions)
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<center><h1>Subculturing E. Coli</h1></center> | <center><h1>Subculturing E. Coli</h1></center> | ||
<br/> | <br/> | ||
- | What you need: <br/> | + | What you need: <br/><br/> |
- Autoclaved 250 mL flasks <br/> | - Autoclaved 250 mL flasks <br/> | ||
- Liquid Media <br/> | - Liquid Media <br/> | ||
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Procedure: <br/><br/> | Procedure: <br/><br/> | ||
- | 1. Into your autoclaved flask add:<br/> | + | 1. Into your autoclaved flask add:<br/><br/> |
- 50 mL liquid media <br/> | - 50 mL liquid media <br/> | ||
- 50 ul antibiotic solution if necessary <br/> | - 50 ul antibiotic solution if necessary <br/> | ||
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2. Incubate in the 37˚C warm room on a stirrer for 4-6 hours <br/><br/> | 2. Incubate in the 37˚C warm room on a stirrer for 4-6 hours <br/><br/> | ||
- | Note: Need to check the cells 2 hours after inoculation to test their optical density (OD). The best competent cells come from cells in exponential phase of their growth. Therefore an OD of 0.4-0.8 is desired and this happens after 2-4 hours of incubation. <br/> | + | Note: Need to check the cells 2 hours after inoculation to test their optical density (OD). The best competent cells come from cells in exponential phase of their growth. Therefore an OD of 0.4-0.8 is desired and this happens after 2-4 hours of incubation. <br/><br/> |
</div> | </div> |
Latest revision as of 15:31, 4 June 2012