Team:UIUC-Illinois/Notebook/Protocols/Storage

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<center><h1>Protocol Overview</h1></center>
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<center><h1>Storing cells in Glycerol Solution</h1></center><br/>
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What you need:<br/><br/>
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- 1.5 mL cryogenic vials<br/>
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- 50% glycerol solution (can also use 20%) <br/>
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- An overnight culture of the cells of interest<br/><br/>
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Procedure:<br/><br/>
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1. To a cryogenic vial, add 600 uL 50% glycerol solution and 400 uL liquid cell culture<br/>
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2. Mix thoroughy and store in the -80˚C freezer until use <br/><br/>
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Unless noted, <a href="http://www.scs.illinois.edu/rao/index.php">all procedures are based on those in the lab of Christopher Rao</a>. <br/><br/>
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Latest revision as of 15:29, 4 June 2012

Header

Protocols

Select Protocol

Storing cells in Glycerol Solution


What you need:

- 1.5 mL cryogenic vials
- 50% glycerol solution (can also use 20%)
- An overnight culture of the cells of interest

Procedure:

1. To a cryogenic vial, add 600 uL 50% glycerol solution and 400 uL liquid cell culture
2. Mix thoroughy and store in the -80˚C freezer until use

Unless noted, all procedures are based on those in the lab of Christopher Rao.

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