Team:UIUC-Illinois/Notebook/Protocols/Bootcamp
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(Difference between revisions)
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<h2>Making LB for plates</h2> <br/> | <h2>Making LB for plates</h2> <br/> | ||
- | To make 1 Liter of LB: | + | To make 1 Liter of LB: <br/> |
1. Always use a 10:5:5 ratio of Tryptone to Yeast Extract to NaCl. <br/> | 1. Always use a 10:5:5 ratio of Tryptone to Yeast Extract to NaCl. <br/> | ||
2. Add dry ingredients first. <<br/> | 2. Add dry ingredients first. <<br/> | ||
3. Use a 2L flask. <br/> | 3. Use a 2L flask. <br/> | ||
- | 4. Add the following | + | 4. Add the following <br/> |
- 10 g Tryptone <br/> | - 10 g Tryptone <br/> | ||
- 5 g Yeast Extract <br/> | - 5 g Yeast Extract <br/> | ||
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<h2>Making Glycerol Stock </h2> <br/> | <h2>Making Glycerol Stock </h2> <br/> | ||
- | To make 400 mL of 10% glycerol you will need: | + | To make 400 mL of 10% glycerol you will need:<br/> |
-40 mL glycerol<br/> | -40 mL glycerol<br/> | ||
-360 mL of MilliQ water <br/><br/> | -360 mL of MilliQ water <br/><br/> | ||
- | To make 400 mL of 20% glycerol you will need: | + | To make 400 mL of 20% glycerol you will need:<br/> |
-80 mL glycerol<br/> | -80 mL glycerol<br/> |
Revision as of 19:48, 1 June 2012