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Team:UIUC-Illinois/Notebook/Protocols/TAE
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<div id="protocoloverview"> | <div id="protocoloverview"> | ||
| - | <center><h1> | + | <center><h1>Buffer Protocols</h1></center> |
| + | |||
| + | <h2>Making 50X TAE buffer</h2><br/> | ||
| + | |||
| + | What you need: <br/><br/> | ||
| + | - Tris base<br/> | ||
| + | - Glacial acetic acid<br/> | ||
| + | - Na2EDTA•2H2O<br/> | ||
| + | - MilliQ water<br/><br/> | ||
| + | |||
| + | Procedure:<br/><br/> | ||
| + | 1. In a 1000mL pyrex bottle add and mix: | ||
| + | - 242 Tris base<br/> | ||
| + | - 57.1mL glacial acetic acid<br/> | ||
| + | - 37.2g Na2EDTA•2H2O<br/> | ||
| + | - 1000mL MilliQ water<br/> | ||
| + | 2. Mix thoroughly, no sterilization necessary<br/><br/> | ||
| + | |||
| + | <h2>Making 1X TAE buffer</h2><br/> | ||
| + | |||
| + | What you need: <br/> | ||
| + | - 50X TAE buffer <br/> | ||
| + | - MilliQ water <br/><br/> | ||
| + | |||
| + | Procedure:<br/><br/> | ||
| + | |||
| + | 1. Add 20mL 50X TAE buffer to 980mL MilliQ water and mix to make 1L of 1X TAE buffer stock <br/><br/> | ||
</div> | </div> | ||
Revision as of 19:42, 1 June 2012
