Team:UIUC-Illinois/Notebook/Protocols/GelElectrophoresis

From 2012.igem.org

(Difference between revisions)
Line 74: Line 74:
<center><h1>Making Gel Electrophoresis gel solutions</h1></center> <br/><br/>
<center><h1>Making Gel Electrophoresis gel solutions</h1></center> <br/><br/>
-
<h2>To check PCR results</h2><br/><br/>
+
<h2>To check PCR results</h2><br/>
What you need:<br/>
What you need:<br/>
Line 85: Line 85:
3. BE CAREFUL AS LIQUID CAN BE SUPERHEATED<br/><br/>
3. BE CAREFUL AS LIQUID CAN BE SUPERHEATED<br/><br/>
-
<h2>To do a gel purification</h2><br/><br/>
+
<h2>To do a gel purification</h2><br/>
What you need:<br/>
What you need:<br/>
- Low-melt agarose<br/>
- Low-melt agarose<br/>

Revision as of 19:38, 1 June 2012

Header

Protocols

Select Protocol

Making Gel Electrophoresis gel solutions



To check PCR results


What you need:
- High-melt agarose
- TAE buffer 1X

Procedure:
1. Add 3.2g high-melt agarose to 400 mL 1X TAE buffer (50 uL agarose gel, 250 mL 1X TAE buffer for the small gel box)
2. Microwave flask or bottle (cap placed loosely if bottle) of mixed solution to dissolve gel in 1 minute increments until all agarose is dissolved 3. BE CAREFUL AS LIQUID CAN BE SUPERHEATED

To do a gel purification


What you need:
- Low-melt agarose
- TAE buffer 1X

Procedure:
1. Add 4.0g low-melt agarose to 400 mL 1X TAE buffer
2. Microwave flask or bottle (cap placed loosely if bottle) of mixed solution to dissolve gel in 1 minute increments until all agarose is dissolved
3. BE CAREFUL AS LIQUID CAN BE SUPERHEATED

Retrieved from "http://2012.igem.org/Team:UIUC-Illinois/Notebook/Protocols/GelElectrophoresis"