Team:UIUC-Illinois/Notebook/Protocols/Bootcamp
From 2012.igem.org
(Difference between revisions)
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<h2>Ligation </h2><br/> | <h2>Ligation </h2><br/> | ||
- We used the <a href="http://ginkgobioworks.com/support/BioBrick_Assembly_Manual.pdf">Ginkgo bioworks protocol</a> <br/><br/> | - We used the <a href="http://ginkgobioworks.com/support/BioBrick_Assembly_Manual.pdf">Ginkgo bioworks protocol</a> <br/><br/> | ||
- | + | 1. Control: <br/> | |
- | + | - 2 uL of Circular PSB1C3 plasmid 13 uL ddH2O <br/> | |
- | + | - 2 uL T4 ligase buffer<br/> | |
+ | - 1 uL T4 ligase <br/><br/> | ||
+ | 2. P&C: <br/> | ||
+ | - 2 uL of Circular PSB1C3 plasmid<br/> | ||
+ | - 2 uL of PUF, 11 uL ddH2O<br/> | ||
+ | - 2 uL T4 ligase buffer<br/> | ||
+ | - 1 uL T4 ligase<br/><br/> | ||
+ | 3. L&P:<br/> | ||
+ | - 2 uL of linear PSB1C3 plasmid<br/> | ||
+ | - 2 uL of PUF<br/> | ||
+ | - 11 uL ddH2O<br/> | ||
+ | - 2 uL T4 ligase buffer<br/> | ||
+ | - 1 uL T4 ligase<br/><br/> | ||
Revision as of 19:24, 1 June 2012