Team:UIUC-Illinois/Notebook/Protocols/Bootcamp
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(Difference between revisions)
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<h2>Making Glycerol Stock </h2> | <h2>Making Glycerol Stock </h2> | ||
- | To make 400 mL of 10% glycerol you will need: | + | To make 400 mL of 10% glycerol you will need:<br/><br/> |
- | -40 mL glycerol | + | -40 mL glycerol<br/><br/> |
- | -360 mL of MilliQ water | + | -360 mL of MilliQ water <br/><br/> |
- | To make 400 mL of 20% glycerol you will need: | + | To make 400 mL of 20% glycerol you will need:<br/><br/> |
- | -80 mL glycerol | + | -80 mL glycerol<br/> |
- | -320 mL of MilliQ water | + | -320 mL of MilliQ water <br/><br/> |
1. Use flasks and bottles before graduated cylinders (they take forever to mix!) <br/><br/> | 1. Use flasks and bottles before graduated cylinders (they take forever to mix!) <br/><br/> | ||
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<h2>Ligation </h2> | <h2>Ligation </h2> | ||
- | + | - We used the <a href="http://ginkgobioworks.com/support/BioBrick_Assembly_Manual.pdf">Ginkgo bioworks protocol</a> <br/><br/> | |
- | + | - Control: 2 uL of Circular PSB1C3 plasmid, 13 uL ddH2O, 2 uL T4 ligase buffer, 1 uL T4 ligase <br/><br/> | |
- | P&C: 2 uL of Circular PSB1C3 plasmid, 2 uL of PUF, 11 uL ddH2O, 2 uL T4 ligase buffer, 1 uL T4 ligase | + | - P&C: 2 uL of Circular PSB1C3 plasmid, 2 uL of PUF, 11 uL ddH2O, 2 uL T4 ligase buffer, 1 uL T4 ligase <br/><br/> |
- | L&P: 2 uL of linear PSB1C3 plasmid, 2 uL of PUF, 11 uL ddH2O, 2 uL T4 ligase buffer, 1 uL T4 ligase | + | - L&P: 2 uL of linear PSB1C3 plasmid, 2 uL of PUF, 11 uL ddH2O, 2 uL T4 ligase buffer, 1 uL T4 ligase <br/><br/> |
Revision as of 19:11, 1 June 2012