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Team:Evry/Notebook/w8
From 2012.igem.org
(Difference between revisions)
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Digestion of psc2+ and mRFP with EcoRI and PstI.<br/> | Digestion of psc2+ and mRFP with EcoRI and PstI.<br/> | ||
Ligation and Transformation on T10. | Ligation and Transformation on T10. | ||
| + | |||
| + | <h3>TirI BB</h3> | ||
| + | Electrophoresis of TirI last PCR: failure of this technique.<br> | ||
| + | We buy a new primer to try another PCR technic. | ||
<h2>Tuesday, 31st July</h2> | <h2>Tuesday, 31st July</h2> | ||
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Electrophoresis of 27/07 results: Failure of second PCR</br> | Electrophoresis of 27/07 results: Failure of second PCR</br> | ||
We restart the first step | We restart the first step | ||
| + | |||
| + | <h2>Thursday, 2nd August</h2> | ||
| + | |||
| + | <h3>Sequencing</h3> | ||
| + | Send pCS2+ to sequencing. | ||
| + | |||
| + | <h3>Issue</h3> | ||
| + | We found that pCS2+ was badly made. We restart the construction of this plasmid from the beginning. | ||
</html> | </html> | ||
Revision as of 08:03, 3 August 2012
Weeks
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 |
Week 8: 30th July - 5th August
Monday, 30th July
Digestion - Ligation - Transformation:
Digestion of psc2+ and mRFP with EcoRI and PstI.Ligation and Transformation on T10.
TirI BB
Electrophoresis of TirI last PCR: failure of this technique.We buy a new primer to try another PCR technic.
Tuesday, 31st July
Inoculation:
Inoculation of pcs2+ (27.07) and repiquage of psc2+ with RFP (30.07)Inoculation of psB1C3 with GFP-AID TirI: Electrophoresis of 27/07 results: Failure of second PCR We restart the first step
