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Team:NTNU Trondheim/Stocks
From 2012.igem.org
(Difference between revisions)
m (→Isolated DNA) |
(→Isolated DNA) |
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|YFP+Term||11.07||?|YFP+term (religated)||?|| | |YFP+Term||11.07||?|YFP+term (religated)||?|| | ||
|- | |- | ||
| - | |DLD + RBS||9.07||BBa_K284003 + BBa_B0034 || 24,1|| | + | |DLD + RBS||9.07||BBa_K284003 + BBa_B0034 || 24,1||pSB1A2 |
|- | |- | ||
| - | |VHB + RBS||9.07||BBa_K258005 + BBa_B0034 || 48,3|| | + | |VHB + RBS||9.07||BBa_K258005 + BBa_B0034 || 48,3||pSB1A2 |
|- | |- | ||
| - | |Jen1 + RBS||9.07||BBa_K284002 + BBa_B0034 || 35,3|| | + | |Jen1 + RBS||9.07||BBa_K284002 + BBa_B0034 || 35,3||pSB1A2 |
|- | |- | ||
| - | |VGB + RBS||9.07||BBa_K561001 + BBa_B0034 || 40,0|| | + | |VGB + RBS||9.07||BBa_K561001 + BBa_B0034 || 40,0||pSB1A2 |
|- | |- | ||
| - | |LR||?||<partinfo> | + | |LR||?||<partinfo>BBa_C0062</partinfo> ||20,3||pSB1A2 |
|- | |- | ||
|VGB promoter||27.06||<partinfo>BBa_K561001</partinfo>||22,9||pSB1C3 | |VGB promoter||27.06||<partinfo>BBa_K561001</partinfo>||22,9||pSB1C3 | ||
| Line 66: | Line 66: | ||
|Constitutive promoter||22.06||<partinfo>BBa_J23119</partinfo> ||27,7||pSB1A2 | |Constitutive promoter||22.06||<partinfo>BBa_J23119</partinfo> ||27,7||pSB1A2 | ||
|- | |- | ||
| - | |DLD||4.07||<partinfo>BBa_K284003</partinfo>||63,8||BBa_J63010 | + | |DLD||4.07||<partinfo>BBa_K284003</partinfo>||63,8||<partinfo>BBa_J63010</partinfo> |
|- | |- | ||
|K+RBS||13.07||<partinfo>BBa_K081005</partinfo>||19,8||pSB1A2 | |K+RBS||13.07||<partinfo>BBa_K081005</partinfo>||19,8||pSB1A2 | ||
| Line 74: | Line 74: | ||
|pBAD+lysis||9.7|| ||23,6||pSB1A3 | |pBAD+lysis||9.7|| ||23,6||pSB1A3 | ||
|- | |- | ||
| - | |DNA lysis||24.06|| || || | + | |DNA lysis||24.06||<partinfo>BBa_K112808</partinfo> || ||pSB1A2 |
|- | |- | ||
| - | |D2||22.6||BBa_K145150||23,1||pSB1A2 | + | |D2||22.6||<partinfo>BBa_K145150</partinfo>||23,1||pSB1A2 |
|- | |- | ||
|RBS||6.07||<partinfo>BBa_B0034</partinfo>||44,5||pSB1A2 | |RBS||6.07||<partinfo>BBa_B0034</partinfo>||44,5||pSB1A2 | ||
| Line 86: | Line 86: | ||
|Jen||4.07||<partinfo>BBa_K284002</partinfo>||102,1|| | |Jen||4.07||<partinfo>BBa_K284002</partinfo>||102,1|| | ||
|- | |- | ||
| - | |luxR+HSL||25.06|| ||13,3|| | + | |luxR+HSL||25.06||<partinfo>BBa_R0062</partinfo> ||13,3||pSB1A2 |
|- | |- | ||
| - | |Lysis1 DNA||24.06|| || || | + | |Lysis1 DNA||24.06||<partinfo>BBa_K112808</partinfo> || ||pSB1A2 |
|- | |- | ||
|GFP+DTT||13.07||BBa_K082003+BBa_B0015||41,1||pSB1AK3 | |GFP+DTT||13.07||BBa_K082003+BBa_B0015||41,1||pSB1AK3 | ||
| Line 94: | Line 94: | ||
|YFP+DTT||13.07||BBa_E0030+BBa_B0015||55,5||pSB1AK3 | |YFP+DTT||13.07||BBa_E0030+BBa_B0015||55,5||pSB1AK3 | ||
|- | |- | ||
| - | |RFP+DTT||13.07||BBa_E1010+BBa_B0015||40,9|| | + | |RFP+DTT||13.07||BBa_E1010+BBa_B0015||40,9||pSB1AK3 |
|- | |- | ||
| - | |CFP+DTT||13.07||BBa_E0020+BBa_B0015||47,7|| | + | |CFP+DTT||13.07||BBa_E0020+BBa_B0015||47,7||pSB1AK3 |
|- | |- | ||
| - | |pBAD B1||1.07||<partinfo> | + | |pBAD B1||1.07||<partinfo>BBa_K206000</partinfo>||24,0||pSB1A3 |
|- | |- | ||
|D1||22.06||<partinfo>BBa_K091146</partinfo>||19,8||pSB1A2 | |D1||22.06||<partinfo>BBa_K091146</partinfo>||19,8||pSB1A2 | ||
| Line 104: | Line 104: | ||
|pBAD B2||1.07||<partinfo>Bba_K206000</partinfo>||19||pSB1A3 | |pBAD B2||1.07||<partinfo>Bba_K206000</partinfo>||19||pSB1A3 | ||
|- | |- | ||
| - | |Colicin||4.07||<partinfo>BBa_K150009</partinfo>||58,6|| | + | |Colicin||4.07||<partinfo>BBa_K150009</partinfo>||58,6||pSB1A2 |
|- | |- | ||
|VGB+RBS+YFP+DTT||26.07|| || ||pSB1AK3 | |VGB+RBS+YFP+DTT||26.07|| || ||pSB1AK3 | ||
| Line 120: | Line 120: | ||
|DTT Kan||26.06||<partinfo>BBa_B0015</partinfo>||26,5||pSB1AK3 | |DTT Kan||26.06||<partinfo>BBa_B0015</partinfo>||26,5||pSB1AK3 | ||
|- | |- | ||
| - | |RFP K||22.06||BBa_E1010 || ||pSB2K3 | + | |RFP K||22.06||<partinfo>BBa_E1010</partinfo> || ||pSB2K3 |
|- | |- | ||
| - | |GFP AK||22.06||BBa_K082003 || ||pSB1AK3 | + | |GFP AK||22.06||<partinfo>BBa_K082003</partinfo> || ||pSB1AK3 |
|- | |- | ||
| - | |CFP A||22.06||BBa_E0020|| ||pSB1A2 | + | |CFP A||22.06||<partinfo>BBa_E0020</partinfo>|| ||pSB1A2 |
|- | |- | ||
| - | |YFP AK||22.06 ||BBa_E0030||pSB1AK3 | + | |YFP AK||22.06 ||<partinfo>BBa_E0030</partinfo>||pSB1AK3 |
|} | |} | ||
Revision as of 19:11, 28 July 2012
This page lists stored cultures and other samples:
See also Constructs page.
Resuspended DNA
(Dried DNA from distribution kit resuspended in dH2O)
| Name/abbreviation | Date stored | Description | Source | Backbone |
|---|---|---|---|---|
| LR | ? | <partinfo>BBa_C0062</partinfo> | (RS) | pSB1A2 |
| Lysis | 22.06 | BBa_K112808 | (RS) | pSB1A2 |
| C78 | ? | <partinfo>BBa_C0078</partinfo> | (RS) | |
| C79 | ? | <partinfo>BBa_C0079</partinfo> | (RS) | |
| RBS | 24.06 | <partinfo>BBa_B0034</partinfo> | (RS) | pSB1A2 |
| LuxI | ? | <partinfo>BBa_C0061</partinfo> | (RS) | |
| K+RBS | ? | <partinfo>BBa_K081005</partinfo> | (RS) | pSB1A2 |
Isolated DNA
(Miniprep, gel extraction and PCR products)
| Name/abbreviation | Date stored | Description | Concentration | Backbone |
|---|---|---|---|---|
| LuxR+Term | 16.7 | BBa_C0062 + BBa_B0015 | 37,0 | pSB1AK3 |
| LuxI+Term | 16.7 | BBa_C0061 + BBa_B0015 | 37,6 | pSB1AK3 |
| C79 | 27.06 | <partinfo>BBa_C0079</partinfo> | 70,8 | pSB2K3 |
| C78 | 27.06 | <partinfo>BBa_C0078</partinfo> | 63,4 | pSB2K3 |
| RBS | 27.06 | <partinfo>BBa_B0034</partinfo> | 30,2 | pSB1A2 |
| GFP+Term | 11.07 | BBa_K082003 + BBa_B0015 | pSB1AK3 | |
| CFP+Term | 11.07 | BBa_E0020 + BBa_B0015 | pSB1AK3 | |
| LI (BBa_K092400) | ? | <partinfo>BBa_K092400</partinfo> | pSB1A2 | |
| YFP+Term | 11.07 | YFP+term (religated) | ? | |
| DLD + RBS | 9.07 | BBa_K284003 + BBa_B0034 | 24,1 | pSB1A2 |
| VHB + RBS | 9.07 | BBa_K258005 + BBa_B0034 | 48,3 | pSB1A2 |
| Jen1 + RBS | 9.07 | BBa_K284002 + BBa_B0034 | 35,3 | pSB1A2 |
| VGB + RBS | 9.07 | BBa_K561001 + BBa_B0034 | 40,0 | pSB1A2 |
| LR | ? | <partinfo>BBa_C0062</partinfo> | 20,3 | pSB1A2 |
| VGB promoter | 27.06 | <partinfo>BBa_K561001</partinfo> | 22,9 | pSB1C3 |
| VGB DNA | 25.06 | <partinfo>BBa_K561001</partinfo> | pSB1C3 | |
| RFP +Term | 11.07 | <partinfo>Bba_E1010</partinfo>+<partinfo>BBa_B0015</partinfo> | pSB1AK3 | |
| Constitutive promoter | 22.06 | <partinfo>BBa_J23119</partinfo> | 27,7 | pSB1A2 |
| DLD | 4.07 | <partinfo>BBa_K284003</partinfo> | 63,8 | <partinfo>BBa_J63010</partinfo> |
| K+RBS | 13.07 | <partinfo>BBa_K081005</partinfo> | 19,8 | pSB1A2 |
| LuxR+HSL | 24.06 | <partinfo>BBa_R0062</partinfo> | pSB1A2 | |
| pBAD+lysis | 9.7 | 23,6 | pSB1A3 | |
| DNA lysis | 24.06 | <partinfo>BBa_K112808</partinfo> | pSB1A2 | |
| D2 | 22.6 | <partinfo>BBa_K145150</partinfo> | 23,1 | pSB1A2 |
| RBS | 6.07 | <partinfo>BBa_B0034</partinfo> | 44,5 | pSB1A2 |
| LuxI | 6.07 | 38,6 | ||
| VHB | 4.07 | <partinfo>BBa_K258005</partinfo> | 44,9 | pSB1A2 |
| Jen | 4.07 | <partinfo>BBa_K284002</partinfo> | 102,1 | |
| luxR+HSL | 25.06 | <partinfo>BBa_R0062</partinfo> | 13,3 | pSB1A2 |
| Lysis1 DNA | 24.06 | <partinfo>BBa_K112808</partinfo> | pSB1A2 | |
| GFP+DTT | 13.07 | BBa_K082003+BBa_B0015 | 41,1 | pSB1AK3 |
| YFP+DTT | 13.07 | BBa_E0030+BBa_B0015 | 55,5 | pSB1AK3 |
| RFP+DTT | 13.07 | BBa_E1010+BBa_B0015 | 40,9 | pSB1AK3 |
| CFP+DTT | 13.07 | BBa_E0020+BBa_B0015 | 47,7 | pSB1AK3 |
| pBAD B1 | 1.07 | <partinfo>BBa_K206000</partinfo> | 24,0 | pSB1A3 |
| D1 | 22.06 | <partinfo>BBa_K091146</partinfo> | 19,8 | pSB1A2 |
| pBAD B2 | 1.07 | <partinfo>Bba_K206000</partinfo> | 19 | pSB1A3 |
| Colicin | 4.07 | <partinfo>BBa_K150009</partinfo> | 58,6 | pSB1A2 |
| VGB+RBS+YFP+DTT | 26.07 | pSB1AK3 | ||
| VHB+RBS+YFP+DTT | 26.07 | pSB1AK3 | ||
| EYFP+RBS+YFP+DTT | 26.07 | |||
| pTet GFP | 26.07 | <partinfo>BBa_I13522</partinfo> | pSB1A2 | |
| RBS | 26.06 | <partinfo>BBa_B0034</partinfo> | 16,8 | pSB1A2 |
| DTT Amp | 26.06 | <partinfo>BBa_B0015</partinfo> | 35,8 | pSB1AK3 |
| DTT Kan | 26.06 | <partinfo>BBa_B0015</partinfo> | 26,5 | pSB1AK3 |
| RFP K | 22.06 | <partinfo>BBa_E1010</partinfo> | pSB2K3 | |
| GFP AK | 22.06 | <partinfo>BBa_K082003</partinfo> | pSB1AK3 | |
| CFP A | 22.06 | <partinfo>BBa_E0020</partinfo> | pSB1A2 | |
| YFP AK | 22.06 | <partinfo>BBa_E0030</partinfo> | pSB1AK3 |
Glycerol stocks
(Cell cultures kept at -80 C)
| Name/abbreviation | Date stored | Strain | Plasmid | Position |
|---|---|---|---|---|
| pTet mRFP (BBa_I13521) | 27.07 | DH5a | pSB1A2 | |
| pTet GFP (BBa_I13522) | 27.07 | DH5a | pSB1A2 | |
| EYFP + LVA (BBa_E0430) | 27.07 | DH5a | pSB1A2 | |
| RFP (<partinfo>BBa_E1010</partinfo>) | 18.07 | DH5a | pSB2K3 | |
| VGB (<partinfo>BBa_K561001</partinfo>) | 18.07 | DH5a | pSB1C3 | |
| LuxR+HSL (<partinfo>BBa_R0062</partinfo>) | 16.07 | DH5a | pSB1A2 | |
| Lysis (<partinfo>BBa_K112808</partinfo>) | 16.07 | DH5a | pSB1A2 | |
| pBAD (<partinfo>Bba_K206000</partinfo>) | 16.07 | DH5a | pSB1A3 | |
| LuxR (<partinfo>BBa_C0062</partinfo>) | 16.07 | DH5a | pSB1A2 | |
| GFP (<partinfo>BBa_K082003</partinfo>) | 16.07 | DH5a | pSB1AK3 | |
| pBAD+Lysis | 16.07 | DH5a | pSB1A3 | |
| DTT (<partinfo>BBa_B0015</partinfo> | 17.07 | DH5a | pSB1AK3 | |
| D2 (<partinfo>BBa_K145150</partinfo>) | 17.07 | DH5a | pSB1A2 | |
| CFP (<partinfo>BBa_E0020</partinfo>) | 17.07 | DH5a | pSB1A2 | |
| YFP (<partinfo>BBa_E0030</partinfo>) | 17.07 | DH5a | pSB1AK3 |
