Team:Freiburg

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|The iGEM Team Freiburg 2012 (FreiGEM) is dedicated to standardizing the synthesis of Transactivator-like (TAL) proteins, i.e. DNA-binding proteins. In contrast to zinc-finger proteins they consist of domain-like repeats in their primary structure that differ only by two amino acids. These two amino acids of a given repeat determine which DNA base the repeat will bind to. By providing efficient mechanisms to combine the different repeats in order to target a certain DNA sequence of around 14bp the FreiGEM team is working towards establishing a standardized kit. This one tube essay will provide the user with an easy, quick and cost-efficient way to design his or her individualized TAL protein in just a glance.
 
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''Stay tuned to FreiGEM 2012 - we will keep you in the loop.''
 
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[[Team:Freiburg/Team|<span style="color:#2244AA;">The FreiGEM 2012 Team</span>]]
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<h1>Let us tell you a fabulous TALE...</h1>
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Transactivator-Like Effectors (TALEs) are a brand-new technology that currently revolutionizes the way researchers manipulate DNA with exceptional site specificity. Originally derived from ''Xanthomonas spp.'', this type of protein comprizes an effector domain and a modular DNA binding domain that can be rationally designed to bind to virtually any target sequence of DNA. Over the past two years, universal endonucleases (TALENs) and transcription factors have been tested in various organisms ranging from bacteria to humans. According to existing protocols, TALE assembly requires several weeks of work and substantial lab skills. In order to bring this technology within reach for iGEM students, we invented an extremely fast and easy TALE assembly strategy and developed a TALE platform with expression plasmids and new classes of TALEs. With our so called GATE assembly kit, future iGEM students will be able to precisely manipulate genomic loci easier and faster than anyone else in the world.
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<h1>European iGEM Jamboree 2012 Amsterdam:</h1>
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Team freiGEM won a gold medal and a special prize for the 'Best New BioBrick Part or Device, Engineered'! Team freiGEM also advanced to World Championship in Boston!
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<p align="center"><A HREF="http://www.flickr.com/photos/igemeurope/8096765971/in/photostream/" target="_blank"><img class="thumbnail"  img src='http://omnibus.uni-freiburg.de/~lb125/won1.png' width="75%" height="75%"/></A></p>
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<h1><iframe style="margin-left:10px" align="right" width="300" height="250" src="http://player.vimeo.com/video/49902809" frameborder="0" webkitAllowFullScreen mozallowfullscreen allowFullScreen></iframe>Watch our freiGEM movie...<h1>
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<br>
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<h1>...or get some impressions in the freiGEM Photo Gallery:</h1>
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<p><a style="font-weight:bold; font-size:1.3em;" href="/Team:Freiburg/gallery">FreiGEM 2012 Gallery</a></p>
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<div class="slide">
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<h1><iframe style="margin-right:10px" align="left" width="300" height="250" src="http://player.vimeo.com/video/52254697?badge=0" frameborder="0" webkitAllowFullScreen mozallowfullscreen allowFullScreen></iframe>See how easy it is to get your own custom made TAL...</h1>
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<br><br>
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<h1>...for further information visit our project page:</h1>
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<p><a style="font-weight:bold; font-size:1.3em;" href="/Team:Freiburg/Project">Overview TAL Project</a></p>
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<h1>Check out our modeling:</h1>
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<p align="center"><A HREF="/Team:Freiburg/Modeling"><img class="thumbnail"  img src='https://static.igem.org/mediawiki/2012/9/99/FreigemCompanelDNAlogo.png' width="60%" /></A></p>
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Companel|DNA is dedicated to bringing biology closer to quantitative predictitive science.
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<p><a style="font-weight:bold; font-size:1.1em;" href="/Team:Freiburg/Modeling">Let our application help you to clear up complicated or codepending effects in signaling or protein interaction for instance...</a></p>
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<span style="color:#4F8DDE; font-weight:bold;">
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With kind support of
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<A HREF="http://www.bioss.uni-freiburg.de/cms/index.php" target="_blank"><img class="thumbnail"  img src="https://static.igem.org/mediawiki/2012/7/7b/Logo_bioss.gif" width= "200" /></A>
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<A HREF="http://www.genscript.com/" target="_blank"><img class="thumbnail"  img src='https://static.igem.org/mediawiki/2012/0/0d/Genscript_logo.gif' width= "180"/></A>
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<A HREF="http://eu.idtdna.com/site" target="_blank"><img class="thumbnail"  img src='https://static.igem.org/mediawiki/2012/2/21/IDTLogo2010.png' width= "200"/></A>
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<!--- The Mission, Experiments --->
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!align="center"|[[Team:Freiburg|<span style="color:#4169E1;">Home</span>]]
 
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!align="center"|[[Team:Freiburg/Team|<span style="color:#4F8DDE;">Team</span>]]
 
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!align="center"|[https://igem.org/Team.cgi?year=2012&team_name=Freiburg<span style="color:#4F8DDE;">Official Team Profile</span>]
 
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!align="center"|[[Team:Freiburg/Project|<span style="color:#4F8DDE;">Project</span>]]
 
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!align="center"|[[Team:Freiburg/Parts|<span style="color:#4F8DDE;">Parts Submitted to the Registry</span>]]
 
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!align="center"|[[Team:Freiburg/Modeling|<span style="color:#4F8DDE;">Modeling</span>]]
 
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!align="center"|[[Team:Freiburg/Notebook|<span style="color:#4F8DDE;">Notebook</span>]]
 
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!align="center"|[[Team:Freiburg/Safety|<span style="color:#4F8DDE;">Safety</span>]]
 
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Latest revision as of 15:24, 10 November 2012




Let us tell you a fabulous TALE...

Transactivator-Like Effectors (TALEs) are a brand-new technology that currently revolutionizes the way researchers manipulate DNA with exceptional site specificity. Originally derived from ''Xanthomonas spp.'', this type of protein comprizes an effector domain and a modular DNA binding domain that can be rationally designed to bind to virtually any target sequence of DNA. Over the past two years, universal endonucleases (TALENs) and transcription factors have been tested in various organisms ranging from bacteria to humans. According to existing protocols, TALE assembly requires several weeks of work and substantial lab skills. In order to bring this technology within reach for iGEM students, we invented an extremely fast and easy TALE assembly strategy and developed a TALE platform with expression plasmids and new classes of TALEs. With our so called GATE assembly kit, future iGEM students will be able to precisely manipulate genomic loci easier and faster than anyone else in the world.

European iGEM Jamboree 2012 Amsterdam:

Team freiGEM won a gold medal and a special prize for the 'Best New BioBrick Part or Device, Engineered'! Team freiGEM also advanced to World Championship in Boston!

Watch our freiGEM movie...


...or get some impressions in the freiGEM Photo Gallery:

FreiGEM 2012 Gallery

See how easy it is to get your own custom made TAL...



...for further information visit our project page:

Overview TAL Project

Check out our modeling:

Companel|DNA is dedicated to bringing biology closer to quantitative predictitive science.

Let our application help you to clear up complicated or codepending effects in signaling or protein interaction for instance...

Example Frame


With kind support of



Retrieved from "http://2012.igem.org/Team:Freiburg"