"
Team:Nevada/Final Weeks
From 2012.igem.org
(Difference between revisions)
| (3 intermediate revisions not shown) | |||
| Line 29: | Line 29: | ||
<html> | <html> | ||
<br> | <br> | ||
| - | <center><font size=4><b>Final Weeks: October 1- October 31 <font size></b></center> | + | <center><font size=4><b>Final Weeks: October 1- October 31 </font size></b></center> |
</html> | </html> | ||
| - | ==October | + | ==October 20== |
| + | :Jeremiah and Chris: | ||
| + | :::Cultured BL21 cells (glycerol stock) containing the TBP+ insert in the L.A. expression plasmid (added B-complex) | ||
| - | ==October | + | ==October 22== |
| + | :Jeremiah and Chris: | ||
| + | :::Once an OD(600) was measured to be 1.0 the cells were expressed using an L.A. gradient | ||
| + | |||
| + | :::An 8 hour and overnight time sample were taken | ||
| + | |||
| + | ==October 25== | ||
| + | :Jeremiah and Chris: | ||
| + | |||
| + | :::Cells were lysed using a freeze/thaw method to separate the inclusion bodies from the rest of the lysed cell | ||
| + | |||
| + | :::Protein samples were prepared for SDS-PAGE | ||
| + | |||
| + | ==October 26== | ||
| + | :Jeremiah and Chris: | ||
| + | |||
| + | :::Run SDS-PAGE (70 V for 2.5 hrs) | ||
| + | |||
| + | :::Stain gel with coomassie brilliant blue | ||
Latest revision as of 02:03, 27 October 2012
Protocols | Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18 | Week 19 | Final Weeks |
Contents |
October 20
- Jeremiah and Chris:
- Cultured BL21 cells (glycerol stock) containing the TBP+ insert in the L.A. expression plasmid (added B-complex)
October 22
- Jeremiah and Chris:
- Once an OD(600) was measured to be 1.0 the cells were expressed using an L.A. gradient
- An 8 hour and overnight time sample were taken
October 25
- Jeremiah and Chris:
- Cells were lysed using a freeze/thaw method to separate the inclusion bodies from the rest of the lysed cell
- Protein samples were prepared for SDS-PAGE
October 26
- Jeremiah and Chris:
- Run SDS-PAGE (70 V for 2.5 hrs)
- Stain gel with coomassie brilliant blue
