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Team:Nevada/Week 1
From 2012.igem.org
Protocols | Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18 | Week 19 | Final Weeks |
Contents |
May 21
- Michelle, Justin, and Joe:
- Make Buffers P1, P2, N3, PB, and PE for QIAprep Miniprep.
May 22
- Jeremiah and Chris:
- Creation of competent cells and seed stocks. – For use in cloning, recombination, and transformation.
- Michelle, Justin, and Joe:
- Continue making Buffers for QIAprep Miniprep.
May 23
- Jeremiah and Chris:
- Culturing plasmids and digesting inserts out of stock plasmids to insert into SBP plasmid for culturing and recombination. Glycerol stocks of all four plasmids made. Plasmids are cultured in both LB-A broth and on plates.
- SBP with EcoRI and PstI to make backbone
- TBP with XbaI and PstI to make insert
- Culturing plasmids and digesting inserts out of stock plasmids to insert into SBP plasmid for culturing and recombination. Glycerol stocks of all four plasmids made. Plasmids are cultured in both LB-A broth and on plates.
- Michelle, Justin, and Joe:
- Continue making Buffers for QIAprep Miniprep
May 24
- Jeremiah and Chris:
- SBP, VB12, TBP, and Backbone digests
- Digestions were made of TBP using EcoRI, XbaI, and PstI to be used as insert into SBP plasmid cut with SpeI and PstI. Parts were ligated together creating a cloning plasmid with both SBP and TBP.
- Michelle and Joe:
- Miniprep SBP (Starch-Binding Protein) and LRP (Lysine-Rich Protein).
- Digest SBP with enzymes SpeI, and PstI.
- Digest LRP with enzymes PstI, and XbaI.
May 25
- Jeremiah, Joe, Justin, Michelle, and Cody:
- SBP digestions and ligations of other various genes (LRP, Thiamine, Vitamin B12) to SBP individually.
