Team:Nevada/Final Weeks

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<center><font size=4><b>Final Weeks: October 1- October 31 </font size></b></center>
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==October 20==
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:Jeremiah and Chris:
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:::Cultured BL21 cells (glycerol stock) containing the TBP+ insert in the L.A. expression plasmid (added B-complex)
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<!-- You should make use of the calendar feature on the wiki and start a lab notebook.  This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well. -->
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==October 22==
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:Jeremiah and Chris:
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:::Once an OD(600) was measured to be 1.0 the cells were expressed using an L.A. gradient
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:::An 8 hour and overnight time sample were taken
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==October 25==
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:Jeremiah and Chris:
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:::Cells were lysed using a freeze/thaw method to separate the inclusion bodies from the rest of the lysed cell
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:::Protein samples were prepared for SDS-PAGE
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==October 26==
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:Jeremiah and Chris:
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:::Run SDS-PAGE (70 V for 2.5 hrs)
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:::Stain gel with coomassie brilliant blue

Latest revision as of 02:03, 27 October 2012



Protocols | Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18 | Week 19 | Final Weeks |


Final Weeks: October 1- October 31

Contents

October 20

Jeremiah and Chris:
Cultured BL21 cells (glycerol stock) containing the TBP+ insert in the L.A. expression plasmid (added B-complex)

October 22

Jeremiah and Chris:
Once an OD(600) was measured to be 1.0 the cells were expressed using an L.A. gradient
An 8 hour and overnight time sample were taken

October 25

Jeremiah and Chris:
Cells were lysed using a freeze/thaw method to separate the inclusion bodies from the rest of the lysed cell
Protein samples were prepared for SDS-PAGE

October 26

Jeremiah and Chris:
Run SDS-PAGE (70 V for 2.5 hrs)
Stain gel with coomassie brilliant blue

Retrieved from "http://2012.igem.org/Team:Nevada/Final_Weeks"