Team:KAIST Korea/Project Future
From 2012.igem.org
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- | + | <span id="starter">I</span>n mechanical engineering, various devices are designed to operate the machine. Throughout our conceptual and applicable designs, we have tried to demonstrate the possibility of reproducing the notion of mechanical engineering in “Biological Machine”. </br> | |
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Various results from our study show that when the auto-regulatory device is introduced into biological machine, the <i>Escherichia coli </i> cell, it can operate the system as we intended. In application section, we have explained the bio-indigo production system which is operated in accordance with change in AHL concentration. </span></br> | Various results from our study show that when the auto-regulatory device is introduced into biological machine, the <i>Escherichia coli </i> cell, it can operate the system as we intended. In application section, we have explained the bio-indigo production system which is operated in accordance with change in AHL concentration. </span></br> | ||
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Because the binary signal generator produces one out of two proteins with their genes in opposite orientation, we can apply the device to manage two competing pathways. If the resource to operate the pathway we want is a critical factor for cell survival, our device will be helpful for biological machine. By arranging two pathways, one expressing resource protein and the other expressing target protein, in different orientation, we can have auto-regulated pathway which is operated by binary signal generator. </span></br></br> | Because the binary signal generator produces one out of two proteins with their genes in opposite orientation, we can apply the device to manage two competing pathways. If the resource to operate the pathway we want is a critical factor for cell survival, our device will be helpful for biological machine. By arranging two pathways, one expressing resource protein and the other expressing target protein, in different orientation, we can have auto-regulated pathway which is operated by binary signal generator. </span></br></br> | ||
- | <span id=" | + | <span id="little2">The specific example is operating the 1,4-BDO pathway in E.coli. Because the enzymes participating in the 1,4-BDO pathway requires high NADH potential, the critical factor to cell survival, simple cloning of the enzymes may bring harmful effect to the cells. By designing 1,4-BDO enzymes and NADH detector(or redox potential detector) at each end of binary signal generator, we can produce 1,4-BDO effectively without harmful effect to growth of Escherichia coli cells.</span> |
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All iGEM Team KAIST members spent the hottest summer this year. We have learned various experimental techniques, background knowledge and also about “Synthetic biology”. Because none of us had experienced synthetic biology, it took some time to make our theme to contain true synthetic biological meaning. Nevertheless, passionate members of KAIST team were able to reach their own meaning of synthetic biology. </span> | All iGEM Team KAIST members spent the hottest summer this year. We have learned various experimental techniques, background knowledge and also about “Synthetic biology”. Because none of us had experienced synthetic biology, it took some time to make our theme to contain true synthetic biological meaning. Nevertheless, passionate members of KAIST team were able to reach their own meaning of synthetic biology. </span> | ||
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Because we made great efforts to get our concept, we do not want it to fade out after iGEM competition. We hope we can help following-up iGEM teams from KAIST, other teams in Korea, or even any other teams in the World. And we believe our insight to synthetic biology will make our studies more productive and creative, even after iGEM. | Because we made great efforts to get our concept, we do not want it to fade out after iGEM competition. We hope we can help following-up iGEM teams from KAIST, other teams in Korea, or even any other teams in the World. And we believe our insight to synthetic biology will make our studies more productive and creative, even after iGEM. | ||
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Revision as of 22:01, 26 October 2012
2012 KAIST Korea
Mail : kaist.igem.2012@gmail.com
Twitter : twitter.com/KAIST_iGEM_2012
Facebook : www.facebook.com/KAISTiGEM2012
Project : Future Plan