"
Team:Freiburg/Project/Coop
From 2012.igem.org
| (2 intermediate revisions not shown) | |||
| Line 12: | Line 12: | ||
<b>Collaboration with Team Potsdam Bioware:</b> | <b>Collaboration with Team Potsdam Bioware:</b> | ||
| - | Team Potsdam is working on a CHO cell-based project for generating antibodies. Hypermutation should be accomplished by cotransfection of the activation-induced cytidine deaminase (AID). Since it is obviously preferable that the activity of the AID is focussed on the antibody gene, we decided to put our expertise in our two distinct research fields together and produce a TAL-AID fusion protein that binds to the antibody gene-containing plasmid. Using the Plug-and-Play Effector Cassette (PPEC) in our eukaryotic TAL expression vector, it was quite easy to fuse the AID gene to our TAL scaffold. The next step, namely targeting the TAL-AID construct to the antibody plasmid was a child's play with our GATE kit. So we produced a TAL-AID that specifically binds to the sequence T G C G G T C T A T T A C T and currently test it in collaboration with the Potsdam Bioware Team. We hope that we will be able to show you the new data in Boston. | + | Team Potsdam is working on a CHO cell-based project for generating antibodies. Hypermutation should be accomplished by cotransfection of the activation-induced cytidine deaminase (AID). Since it is obviously preferable that the activity of the AID is focussed on the antibody gene, we decided to put our expertise in our two distinct research fields together and produce a TAL-AID fusion protein that binds to the antibody gene-containing plasmid. Using the Plug-and-Play Effector Cassette (PPEC) in our eukaryotic TAL expression vector, it was quite easy to fuse the AID gene to our TAL scaffold. The next step, namely targeting the TAL-AID construct to the antibody plasmid, was a child's play with our GATE kit. So we produced a TAL-AID that specifically binds to the sequence T G C G G T C T A T T A C T and currently test it in collaboration with the Potsdam Bioware Team. We hope that we will be able to show you the new data in Boston. |
| Line 19: | Line 19: | ||
We are in contact with Team Fatih Medical since May and discussed exciting new applications of TAL effectors. Moreover, we have filled out their survey on synthetic biology, which is part of their human practices project. | We are in contact with Team Fatih Medical since May and discussed exciting new applications of TAL effectors. Moreover, we have filled out their survey on synthetic biology, which is part of their human practices project. | ||
| - | + | ||
| - | + | ||
| - | + | ||
<br><br> | <br><br> | ||
<br><br> | <br><br> | ||
[[#top|Back to top]] | [[#top|Back to top]] | ||
Latest revision as of 21:34, 26 October 2012
Collaborations
Collaboration with Team Potsdam Bioware: Team Potsdam is working on a CHO cell-based project for generating antibodies. Hypermutation should be accomplished by cotransfection of the activation-induced cytidine deaminase (AID). Since it is obviously preferable that the activity of the AID is focussed on the antibody gene, we decided to put our expertise in our two distinct research fields together and produce a TAL-AID fusion protein that binds to the antibody gene-containing plasmid. Using the Plug-and-Play Effector Cassette (PPEC) in our eukaryotic TAL expression vector, it was quite easy to fuse the AID gene to our TAL scaffold. The next step, namely targeting the TAL-AID construct to the antibody plasmid, was a child's play with our GATE kit. So we produced a TAL-AID that specifically binds to the sequence T G C G G T C T A T T A C T and currently test it in collaboration with the Potsdam Bioware Team. We hope that we will be able to show you the new data in Boston.
Collaboration with Team Fatih Medical:
We are in contact with Team Fatih Medical since May and discussed exciting new applications of TAL effectors. Moreover, we have filled out their survey on synthetic biology, which is part of their human practices project.
