Team:KAIST Korea/Project Future
From 2012.igem.org
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- | Various | + | Various results from our study show that when the auto-regulatory device is introduced into biological machine, the <i>Escherichia coli cell<i/>, it can operate the system as we intended. In application section, we have explained the bio-indigo production system which is operated in accordance with change in AHL concentration. </span></br> |
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- | Because the binary signal generator | + | Because the binary signal generator produces one out of two proteins with different orientation, we can apply the device to manage two competing pathways. If the resource to operate the pathway we want is a critical factor for cell survival, we can think of applying our device into biological machine. Arranging the genes for resource protein generating pathway and the target pathway genes in different orientation, we can make auto-regulated pathway that is operated by binary signal generator. </span></br></br> |
<span id="little">The specific example can be operating the 1,4-BDO pathway in E.coli. Because the enzymes participating in the 1,4-BDO pathway requires high NADH potential which is critical to cell survival, simple cloning of the enzymes can give harmful effect to the cells. So that, designing 1,4-BDO enzymes and NADH detector(or redox potential detector) at the both end of the binary signal generator, we can produce 1,4-BDO effectively not giving harmful effect to growth of Escherichia coli cells.</span> | <span id="little">The specific example can be operating the 1,4-BDO pathway in E.coli. Because the enzymes participating in the 1,4-BDO pathway requires high NADH potential which is critical to cell survival, simple cloning of the enzymes can give harmful effect to the cells. So that, designing 1,4-BDO enzymes and NADH detector(or redox potential detector) at the both end of the binary signal generator, we can produce 1,4-BDO effectively not giving harmful effect to growth of Escherichia coli cells.</span> | ||
Revision as of 20:06, 26 October 2012
2012 KAIST Korea
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Project : Future Plan