Team:UC Chile/Cyano/Labook/june

From 2012.igem.org

(Difference between revisions)

Latest revision as of 19:15, 26 October 2012

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012

June

Monthly Summary

We are waiting for Gibson enzymes to arrive. So there is no much wet lab work this month.

PCR runs

While waiting for enzymes, all parts for future assemblies are being prepared.

Problematic parts: Kanr, P1003, B0014.

Tried with different DMSO concentrations. Best result at 5%.

Comments: we realized that our current primers work for P1003+B0015. Right primers were now ordered.

After arrival, no amplification for Kanr. Tried once more with an old Kanr purification: success.

New batch of competent cells prepared: Competence: 3.5x10^7 (not quite sure)

Gibson enzymes arrived late June.

@@ Line 30: / Line 30: @@
 <div id="navbar3">
 <ul>Lab book:<br>
-<li><a href="https://2012.igem.org/Team:UC_Chile/Labook/march">March</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/april">April</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/may">May</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/june">June</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/july">July</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/august">August</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/september">September</a></li>
+<li><a href="https://2012.igem.org/Team:UC_Chile/Labook/march">March</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/april">April</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/may">May</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/june">June</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/july">July</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/august">August</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/september">September</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/october">October</a></li>
 </ul>
 </div>
@@ Line 39: / Line 39: @@
-[[File:junelabook_uc_chile.jpg|center]]
+<font size="4">Monthly Summary</font>
+We are waiting for Gibson enzymes to arrive. So there is no much wet lab work this month.
+<font size="4">PCR runs</font>
+While waiting for enzymes, all parts for future assemblies are being prepared.
+Problematic parts: Kanr, P1003, B0014.
+Tried with different DMSO concentrations. Best result at 5%.
+Comments: we realized that our current primers work for P1003+B0015. Right primers were now ordered.
+After arrival, no amplification for Kanr. Tried once more with an old Kanr purification: success.
+New batch of competent cells prepared: Competence: 3.5x10^7 (not quite sure)
+Gibson enzymes arrived late June.
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