Team:UC Chile/Cyano/Labook/june

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<ul>Lab book:<br>
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<li><a href="https://2012.igem.org/Team:UC_Chile/Labook/march">March</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/april">April</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/may">May</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/june">June</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/july">July</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/august">August</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/september">September</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/october">October</a></li>
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<p><font size= "5">June</font></p>
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<font size="4">Monthly Summary</font>
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We are waiting for Gibson enzymes to arrive. So there is no much wet lab work this month.
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<font size="4">PCR runs</font>
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While waiting for enzymes, all parts for future assemblies are being prepared.
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Problematic parts: Kanr, P1003, B0014.
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Tried with different DMSO concentrations. Best result at 5%.
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Comments: we realized that our current primers work for P1003+B0015. Right primers were now ordered.
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After arrival, no amplification for Kanr. Tried once more with an old Kanr purification: success.
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New batch of competent cells prepared: Competence: 3.5x10^7 (not quite sure)
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Gibson enzymes arrived late June.
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Latest revision as of 19:15, 26 October 2012

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012