Team:UC Chile/Cyano/Labook/june

From 2012.igem.org

(Difference between revisions)
(Created page with "{{UC_Chile4}} File:junelabook_uc_chile.jpg")
 
(7 intermediate revisions not shown)
Line 1: Line 1:
{{UC_Chile4}}
{{UC_Chile4}}
 +
<html>
 +
<style type="text/css">
 +
#navbar3 ul{
 +
  list-style-type: none;
 +
  text-align: left;
 +
}
 +
#navbar3 li{
 +
  display: inline;
 +
  text-align: center;
 +
  margin: 0 0 0 0;
 +
}
-
[[File:junelabook_uc_chile.jpg]]
+
#navbar3 li a {
 +
  padding: 2px 7px 2px 7px;
 +
  color: #ffffff;
 +
  background-color: #0000FF;
 +
  text-decoration: none;
 +
}
 +
#navbar3 li a:hover{
 +
  background-color: #333333;
 +
  color: #ffffff;
 +
}
 +
  </style>
 +
</head>
 +
<body>
 +
 
 +
 
 +
<br>
 +
<div id="navbar3">
 +
<ul>Lab book:<br>
 +
<li><a href="https://2012.igem.org/Team:UC_Chile/Labook/march">March</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/april">April</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/may">May</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/june">June</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/july">July</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/august">August</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/september">September</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/october">October</a></li>
 +
</ul>
 +
</div>
 +
</html>
 +
 
 +
 
 +
<p><font size= "5">June</font></p>
 +
 
 +
 
 +
<font size="4">Monthly Summary</font>
 +
 
 +
 
 +
We are waiting for Gibson enzymes to arrive. So there is no much wet lab work this month.
 +
 
 +
 
 +
<font size="4">PCR runs</font>
 +
 
 +
 
 +
While waiting for enzymes, all parts for future assemblies are being prepared.
 +
 
 +
Problematic parts: Kanr, P1003, B0014.
 +
 
 +
Tried with different DMSO concentrations. Best result at 5%.
 +
 
 +
Comments: we realized that our current primers work for P1003+B0015. Right primers were now ordered.
 +
 
 +
After arrival, no amplification for Kanr. Tried once more with an old Kanr purification: success.
 +
 
 +
New batch of competent cells prepared: Competence: 3.5x10^7 (not quite sure)
 +
 
 +
Gibson enzymes arrived late June.
 +
 
 +
{{UC_Chilefooter}}

Latest revision as of 19:15, 26 October 2012

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012