"
Team:UC Chile/Cyanolux/Results short
From 2012.igem.org
Juan Alamos (Talk | contribs) |
|||
| Line 64: | Line 64: | ||
<h3>High-sensitive camera</h3> | <h3>High-sensitive camera</h3> | ||
| - | + | During the Latin America Jamboree, we had a chat with a couple of judges and a student, David Olarte, on how to induce Synechocystis with n-decanal. | |
| - | + | David sent us some work he had done on luminescence assays in cyanobacteria. Following his methods finally we were able to see light emition. | |
| - | + | <html><center><img src="https://static.igem.org/mediawiki/2012/1/1e/Results.post.colombia.camera.jpg" align="left" width="700"></center></html> | |
| + | <br /> | ||
| + | <br /> | ||
| + | <br /> | ||
<h1>Experimental Highlights</h1> | <h1>Experimental Highlights</h1> | ||
Revision as of 04:22, 26 October 2012
Contents |
Construction
Plasmids backbones
We built two integrative plasmid backbones for Synechocystis PCC. 6803.
Constructs for Synechocystis
Using our plasmid backbones we proceeded to build our constructs for Synechocystis (LuxAB & LuxCDEG)
Characterization in Synechocystis
Transformation
We used our plasmid backbones to transform Synechocystis PCC. 6803.
Transformation with pSB1C3_IntK (BBa_K743006):
Transformation with pSB1C3_IntS (BBa_K743010):
Verification of constructs
We proceeded to verificate the integration of the constructs in Synechocystis.
(New image with illustration of verification from outer RS1 and RS2 - October 1X something)
Bioluminescence Assays
Bioluminometer
-graph-
High-sensitive camera
During the Latin America Jamboree, we had a chat with a couple of judges and a student, David Olarte, on how to induce Synechocystis with n-decanal. David sent us some work he had done on luminescence assays in cyanobacteria. Following his methods finally we were able to see light emition.
Experimental Highlights
