"
Team:UC Chile/Cyanolux/Results short
From 2012.igem.org
(Difference between revisions)
| Line 6: | Line 6: | ||
We built two integrative plasmid backbones for Synechocystis PCC. 6803. | We built two integrative plasmid backbones for Synechocystis PCC. 6803. | ||
| - | [[File:UC_Chile-IntKplasmidResult.jpg| | + | [[File:UC_Chile-IntKplasmidResult.jpg| 400px | left]] |
[[File:UC_Chile-IntSplasmidResult.jpg| 480px | right]] | [[File:UC_Chile-IntSplasmidResult.jpg| 480px | right]] | ||
| + | <br /> | ||
| + | <br /> | ||
| + | <br /> | ||
| + | <br /> | ||
| + | <br /> | ||
| + | <br /> | ||
| + | <br /> | ||
<h2>Constructs for Synechocystis</h2> | <h2>Constructs for Synechocystis</h2> | ||
Revision as of 02:02, 26 October 2012
Contents |
Construction
Plasmids backbones
We built two integrative plasmid backbones for Synechocystis PCC. 6803.
Constructs for Synechocystis
Using our plasmid backbones we proceeded to build our constructs for Synechocystis (LuxAB & LuxCDEG)
Characterization in Synechocystis
Transformation
We used our plasmid backbones to transform Synechocystis PCC. 6803.
-Image of Syne plates with colonies-
Verification of constructs
We proceeded to verificate the integration of the constructs in Synechocystis.
(New image with illustration of verification from outer RS1 and RS2 - October 1X something)
Bioluminescence Assays
Bioluminometer
-graph-
High-sensitive camera
-image-
Experimental Highlights
