Team:KAIST Korea/Project Background
From 2012.igem.org
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<img src="https://static.igem.org/mediawiki/2012/9/9f/KAIST_Proj_Overview.png"/></br></br> | <img src="https://static.igem.org/mediawiki/2012/9/9f/KAIST_Proj_Overview.png"/></br></br> | ||
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- | Our new biobrick part <span style="color:#aa0000;"><b>FlipFlop(BBa_K907002 and BBa_K907003)</b> </span> is a noble device which can generate binary signal as its name | + | Our new biobrick part <span style="color:#aa0000;"><b>FlipFlop(BBa_K907002 and BBa_K907003)</b> </span> is a noble device which can generate binary signal as its name implies.</br></br> |
<span id="little">This part is composed of three sub-parts.</br></br></span> | <span id="little">This part is composed of three sub-parts.</br></br></span> | ||
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- | The FlipFlop, initially | + | The FlipFlop, initially promotes the transcription and translation of down-stream gene, Protein A in the Figure 2, according to its promoter orientation. |
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<br><img style="width:340px;" src="https://static.igem.org/mediawiki/2012/a/ae/KAIST_Proj_Overview3_2.PNG"/> | <br><img style="width:340px;" src="https://static.igem.org/mediawiki/2012/a/ae/KAIST_Proj_Overview3_2.PNG"/> |
Revision as of 09:51, 16 October 2012
![](https://static.igem.org/mediawiki/2012/5/59/Logopart_Kaist.png)
![](https://static.igem.org/mediawiki/2012/6/61/Home_kaist_background.png)
2012 KAIST Korea
Mail : kaist.igem.2012@gmail.com
Twitter : twitter.com/KAIST_iGEM_2012
Facebook : www.facebook.com/KAISTiGEM2012
Project : Overview
![](https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png)
![](https://static.igem.org/mediawiki/2012/9/9f/KAIST_Proj_Overview.png)
- Promoter : BBa_J23119
- RBS : BBa_B0034
- att sites : Recognition site for BBa_K907000(Mycobacteriophage Bxb1 integrase).
Signal 0
![](https://static.igem.org/mediawiki/2012/1/10/KAIST_Proj_Overview2.png)
Signal 1
![](https://static.igem.org/mediawiki/2012/1/1e/KAIST_Proj_Overview4.png)
![](https://static.igem.org/mediawiki/2012/4/40/KAIST_Proj_Overview5.png)
Signal 0
![](https://static.igem.org/mediawiki/2012/3/32/KAIST_Proj_Overview6.png)
![](https://static.igem.org/mediawiki/2012/0/0b/KAIST_Overview_2nd_1.png)
![](https://static.igem.org/mediawiki/2012/c/cc/KAIST_Overview_2nd_2.png)
What is bFMO?
Bacterial flavin-containing monooxygenase(bFMO) convert indole, which is produced from primary metabolite of organisms, into isatin, sequentially catalyzed into indigoid compounds that express indigo color.
Because the enzyme utilizing the primary metabolite, we can easily notice the enzyme is working well or not. For the following experiments, engineered bFMO gene from Methylophaga sp. Strain SK1 is kindly provided by Duhee Bang from Yonsei University, Republic of Korea.
![](https://static.igem.org/mediawiki/2012/c/c8/KAIST_Overview_2nd_3.png)
![](https://static.igem.org/mediawiki/2012/b/b9/KAIST_Overview_2nd_4.png)
![](https://static.igem.org/mediawiki/2012/d/d7/KAIST_Overview_2nd_5.png)