Team:UCSF/Auxotroph Data
From 2012.igem.org
(2 intermediate revisions not shown) | |||
Line 31: | Line 31: | ||
- | <img align="left" style="margin-right: | + | <img align="left" style="margin-right:178px;margin-left:68px; margin-bottom:8px; width:500px;height:250px; padding:0;" src="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/auxotrophs/Surface-model.jpg"><br><p> |
- | |||
+ | <p> <regulartext>Based on this model, it seems that a wide variety of ratios of the two strains could be obtained. | ||
+ | <p><br> | ||
+ | |||
+ | <p> <regulartext>So, we performed experiments to determine the ratios of the two strains that could be collected in a variety of inducer conditions. However, based on our results from the YFP standard curve (above) we realized that the data would only be accurate in a small range of cell concentrations. The details of our experimental setup can be found on our Project Protocols page: <a href="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/Protocols/Coculturing%20of%20Auxotrophs.pdf"><b> Co-culturing of Auxotroph Strains </b> </a>. | ||
<p> | <p> | ||
- | <regulartext> A 3D surface of that graph was obtained and a slice of the graph | + | <regulartext> A 3D surface of that graph was obtained and a slice of the graph are shown below: <br> <p> |
+ | <h3> While in some amounts of inducer concentrations, the system seems tunable, it is not nearly as robust as the model predicts. Thus, we turned to our toxin/antitoxin system as a new approach to tuning communities of cells. <br><p> | ||
- | <img align="left" style="margin-bottom: | + | <img align="left" style="margin-bottom:18px; width:555px;height:410px; padding:0;" src="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/auxotrophs/surf-data.png"> |
<img align="left" style="margin-left:168px;margin-bottom:8px; width:555px;height:410px; padding:0;" src="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/auxotrophs/slice.png"> | <img align="left" style="margin-left:168px;margin-bottom:8px; width:555px;height:410px; padding:0;" src="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/auxotrophs/slice.png"> |
Latest revision as of 03:47, 4 October 2012
![UCSF](https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/Logos/Cell%20Mates%20Logo.jpg)
![UCSF](http://dl.dropbox.com/u/24404809/Mission%20Bay.jpg)
While in some amounts of inducer concentrations, the system seems tunable, it is not nearly as robust as the model predicts. Thus, we turned to our toxin/antitoxin system as a new approach to tuning communities of cells.