"
Team:Columbia-Cooper-NYC/Plates
From 2012.igem.org
(Difference between revisions)
| (2 intermediate revisions not shown) | |||
| Line 159: | Line 159: | ||
<div id="m6" onmouseover="mcancelclosetime()" onmouseout="mclosetime()"> | <div id="m6" onmouseover="mcancelclosetime()" onmouseout="mclosetime()"> | ||
<a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Outreach">Outreach</a> | <a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Outreach">Outreach</a> | ||
| - | |||
</div> | </div> | ||
</li> | </li> | ||
| Line 165: | Line 164: | ||
<div id="m7" onmouseover="mcancelclosetime()" onmouseout="mclosetime()"> | <div id="m7" onmouseover="mcancelclosetime()" onmouseout="mclosetime()"> | ||
<a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Safety">Safety</a> | <a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Safety">Safety</a> | ||
| - | + | </div> | |
| - | + | ||
</li> | </li> | ||
<li><a href="#" onmouseover="mopen('m8')" onmouseout="mclosetime()">Sponsors</a> | <li><a href="#" onmouseover="mopen('m8')" onmouseout="mclosetime()">Sponsors</a> | ||
| Line 197: | Line 195: | ||
# Apply 500µl of 1000x stock antibiotics to make 1x solution | # Apply 500µl of 1000x stock antibiotics to make 1x solution | ||
# Pour solution into plates | # Pour solution into plates | ||
| + | |||
| + | Return to [[Team:Columbia-Cooper-NYC/Protocols|Protocols Page]] | ||
Latest revision as of 03:41, 4 October 2012
Protocol for Creating Plates
- The following is the protocol for creating plates to grow bacteria on:
- Prepare 1L bottle
- For 500mL, add 7.5g of agar and 12.5g of LB
- Pour deionized water until water level reaches 500mL
- Cover bottle with foil and tape with autoclave tape
- Autoclave on slow for 20 minutes
- After autoclaving, let bottle stand cool until touch
- Apply 500µl of 1000x stock antibiotics to make 1x solution
- Pour solution into plates
Return to Protocols Page
