Team:Columbia-Cooper-NYC/Electroporation
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Latest revision as of 03:41, 4 October 2012
Electroporation Protocol
- The following is the electroporation protocol provided by bioline:
- Place cuvettes and competent cells into ice
- Add 1µl of DNA to 50µl of competent cells into 1mL cuvettes as quickly as possible
- Keep cuvettes in ice until electroporation
- Electroporate cells at 1800V
- Place bacteria back into tube and add 100-200µl of LB
- Place samples in shaker for 37C for 1 hour
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