Team:Columbia-Cooper-NYC/Chemical Transformation

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= Chemical Transformation/Heat Shock Protocol from Bioline =
= Chemical Transformation/Heat Shock Protocol from Bioline =
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#
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# Remove and thaw chemically competent cells
 +
# Aliquot 1-5µl DNA per 40-50µl of competent cells
 +
# Incubate on ice for 30 minutes
 +
# Heat shock cells for 45 seconds at 42°C
 +
# Let cells stand on ice for 2 minutes
 +
# Place cells in growth media and let it stand for 1 hour
 +
# Plate cells on appropriate antibiotic and add IPTG when appropriate
Return to [[Team:Columbia-Cooper-NYC/Protocols|Protocols Page]]
Return to [[Team:Columbia-Cooper-NYC/Protocols|Protocols Page]]

Latest revision as of 03:39, 4 October 2012


Chemical Transformation/Heat Shock Protocol from Bioline

  1. Remove and thaw chemically competent cells
  2. Aliquot 1-5µl DNA per 40-50µl of competent cells
  3. Incubate on ice for 30 minutes
  4. Heat shock cells for 45 seconds at 42°C
  5. Let cells stand on ice for 2 minutes
  6. Place cells in growth media and let it stand for 1 hour
  7. Plate cells on appropriate antibiotic and add IPTG when appropriate

Return to Protocols Page

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