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Team:Cornell/testing/notebook/wetlab/1
From 2012.igem.org
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<h6>Daily Details:</h6> | <h6>Daily Details:</h6> | ||
| - | + | <br><b>June 27th, Wednesday</b></br> <br>This morning, Steven made glycerol stocks of the 3 Gibson colonies grown overnight. He then did minipreps on the 3 Gibson colonies followed by double digests using PstI and KpnI to check whether the Gibson procedure was successful. | |
| + | Around noon, Steven and Dylan attempted the Myers and Myers Shewanella Electroporation with p17c (pSB3C5). Dylan and Swati then set up a Phusion PCR to amplify the salicylate-sensing region out of BBa_K228227 (to eventually control the expression of mtrB), before running a gel of the restriction digests of the Gibson products. However, our ladder ran strangely, and the gel was determined to be inconclusive in showing that our Gibson colonies appeared to be successful. | ||
| + | That night, Spencer and Steven set up overnight cultures of JG700, nahR, and the 3 Gibson colonies. </br> | ||
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Revision as of 22:31, 3 October 2012
Wet Lab - June
-
Week 1
Having finished our bootcamp, we started working in our lab space in Weill Hall. This week, we began our work to construct our salicylate reporter plasmids using a sensing region including the transcriptional activator nahR. We also continued our work with the Gibson mutagenesis of the nah operon (which degrades naphthalene to salicylate) by attempting to confirm successful construction. We also began our adventure in attempting to transform into Shewanella via electroporation. Daily DetailsWeek 2
It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known. Daily DetailsWeek 3
It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known. Daily DetailsWeek 4
It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known. Daily Details -
Week 1
Having finished our bootcamp, we started working in our lab space in Weill Hall. This week, we began our work to construct our salicylate reporter plasmids using a sensing region including the transcriptional activator nahR. We also continued our work with the Gibson mutagenesis of the nah operon (which degrades naphthalene to salicylate) by attempting to confirm successful construction. We also began our adventure in attempting to transform into Shewanella via electroporation.Week 2
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.Week 3
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.Week 4
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum. -
Week 1
Having finished our bootcamp, we started working in our lab space in Weill Hall. This week, we began our work to construct our salicylate reporter plasmids using a sensing region including the transcriptional activator nahR. We also continued our work with the Gibson mutagenesis of the nah operon (which degrades naphthalene to salicylate) by attempting to confirm successful construction. We also began our adventure in attempting to transform into Shewanella via electroporation.Daily Details:
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.Week 2
It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.Daily Details:
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.Week 3
It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.Daily Details:
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.Week 4
It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.Daily Details:
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
