Team:UCSF/Violacein Results
From 2012.igem.org
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- | <h3red>Violacein Wavelength Scans:</h3red> | + | <h3red>Violacein Mono- and Co-Culture Wavelength Scans:</h3red> |
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Strain 1, which only has the first half of the enzymes necessary to produce violacein is still able to produce a green pigment. When the pigment is extracted from cells, the wavelength scan shown below is obtained. | Strain 1, which only has the first half of the enzymes necessary to produce violacein is still able to produce a green pigment. When the pigment is extracted from cells, the wavelength scan shown below is obtained. |
Revision as of 22:04, 3 October 2012
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Strain 1, which only has the first half of the enzymes necessary to produce violacein is still able to produce a green pigment. When the pigment is extracted from cells, the wavelength scan shown below is obtained.
Strain 2, which has the second half of the violacein pathway, produces no pigment. This is expected because without the first half of the pathway, no pigment can be produced.
Strain 3, which contains the entire violacein operon produces a wavelength scan similar to our standard obtained from Sigma-Aldrich, with violacein having a maximum absorbance near 575nm.