Team:UCSF/Toxin Data
From 2012.igem.org
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<regulartext>We tried several different experiments to determine if the toxin an antitoxin pair could move between cells to cause an effect on growth. The final experiment we performed utilized protein induction at 30C which we believe helped maintain the stability of these small proteins. | <regulartext>We tried several different experiments to determine if the toxin an antitoxin pair could move between cells to cause an effect on growth. The final experiment we performed utilized protein induction at 30C which we believe helped maintain the stability of these small proteins. | ||
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<li> Then toxin, antitoxin, and control cells were inoculated into these filtered, diluted supernatents and induced with IPTG after 2 hours of growth at 37C. </li> | <li> Then toxin, antitoxin, and control cells were inoculated into these filtered, diluted supernatents and induced with IPTG after 2 hours of growth at 37C. </li> | ||
<li> The effect of supernatents potentially containing toxin or antitoxin proteins was observed in the growth of each strain and is shown in the graphs below. </li> | <li> The effect of supernatents potentially containing toxin or antitoxin proteins was observed in the growth of each strain and is shown in the graphs below. </li> | ||
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+ | <regulartext> The graphs shown below demonstrate growth of toxin (YoeB), antitoxin (YefM), and control cells in the media produced after step 5 above. | ||
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+ | <img align="left" style="margin-bottom:8px; width:755px;height:410px; padding:0;" src="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/toxins/FINALcontrolsupgrowth.jpg"> | ||
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+ | <img align="left" style="margin-bottom:8px; width:755px;height:410px; padding:0;" src="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/toxins/FINALantitoxinsupgrowth.jpg"> | ||
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+ | <img align="left" style="margin-bottom:8px; width:755px;height:410px; padding:0;" src="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/toxins/FINALtoxinsupgrowth.jpg"> |
Revision as of 18:37, 3 October 2012