Team:UCSF/Violacein Results
From 2012.igem.org
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- | The details of growth and sample analysis can be found on our protocols page. <p> | + | While other teams and papers have reported that extracting pigment from cultures is the best way to perform analysis and quantification, several different solvents have been reported. The 2009 Cambridge iGEM team used acetone to extract, so we tested extraction of violacen from Strain 3 (full violacein operon) using acetone as well as methanol and ethanol. <p> |
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+ | <img align="left" style="margin-bottom:8px; width:755px;height:410px; padding:0;" src="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/violacein/100112op_solvents.jpg"> | ||
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+ | Our results show that ethanol was the best solvent to use for extraction and so the rest of our extractions were performed in ethanol. The details of growth and sample analysis can be found on our protocols page. <p> | ||
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<img align="left" style="margin-bottom:8px; width:755px;height:410px; padding:0;" src="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/violacein/100112vio_wavelengths.jpg"> | <img align="left" style="margin-bottom:8px; width:755px;height:410px; padding:0;" src="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/violacein/100112vio_wavelengths.jpg"> |
Revision as of 16:48, 3 October 2012
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