Team:UCSF/Violacein Results
From 2012.igem.org
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- | Before we started our experiments, we obtained a violacein standard from Sigma-Aldrich,<a href="http://www.sigmaaldrich.com/catalog/product/sigma/v9389?lang=en®ion=US">Item:V9389-1MG </a><span>. We used this standard to measure the absorbance of violacein at varying concentrations, obtaining the standard curve shown below. <br> | + | Before we started our experiments, we obtained a violacein standard from Sigma-Aldrich,<a href="http://www.sigmaaldrich.com/catalog/product/sigma/v9389?lang=en®ion=US">Item:V9389-1MG </a><span>. We used this standard to measure the absorbance of violacein at varying concentrations, obtaining the standard curve shown below. Full wavelength scans were obtained, but as shown by previous iGEM teams (<a href="https://2009.igem.org/Team:Cambridge/Project/Violacein">Cambridge 2009 </a>) the maximum absorbance of violacein is seen at 575nm and is reported here. <br> |
<img align="left" style="margin-bottom:8px; width:755px;height:410px; padding:0;" src="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/violacein/100212vio_std_cf.jpg"> | <img align="left" style="margin-bottom:8px; width:755px;height:410px; padding:0;" src="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/violacein/100212vio_std_cf.jpg"> | ||
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- | The details of growth and sample analysis can be found on our protocols page. | + | The details of growth and sample analysis can be found on our protocols page. <p> |
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+ | <img align="left" style="margin-bottom:8px; width:755px;height:410px; padding:0;" src="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/violacein/100112vio_wavelengths.jpg"> |
Revision as of 16:39, 3 October 2012
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