Team:UIUC-Illinois/Project/Future/Scaffold
From 2012.igem.org
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<img src="https://static.igem.org/mediawiki/2012/b/bb/WT_PUF-PIN%2C_6-2%2C7-2_PUF-PIN%2C_WT_PUF-aGFP_Lysates.png" height=85% width=85%></center> | <img src="https://static.igem.org/mediawiki/2012/b/bb/WT_PUF-PIN%2C_6-2%2C7-2_PUF-PIN%2C_WT_PUF-aGFP_Lysates.png" height=85% width=85%></center> | ||
- | <br/><b>Fig 1.</b> 1 L culture incubated at 37oC till 0.5 nm optical density after inoculation with 5 mL of overnight for WT PUF-PIN and 6-2/7-2 PUF-PIN. 2 mM IPTG induction for 2 hours. 200 mL cultures incubated at 37oC till 1 nm optical density after inoculation with 2 mL of overnight for WT PUF-αGFP cultures. 2 mM IPTG induction for various hours. | + | <br/><b>Fig. 1.</b> 1 L culture incubated at 37oC till 0.5 nm optical density after inoculation with 5 mL of overnight for WT PUF-PIN and 6-2/7-2 PUF-PIN. 2 mM IPTG induction for 2 hours. 200 mL cultures incubated at 37oC till 1 nm optical density after inoculation with 2 mL of overnight for WT PUF-αGFP cultures. 2 mM IPTG induction for various hours. |
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<img src="https://static.igem.org/mediawiki/2012/4/41/WT_PUF-PIN_Protein_Purification.png" height=85% width=85%></center> | <img src="https://static.igem.org/mediawiki/2012/4/41/WT_PUF-PIN_Protein_Purification.png" height=85% width=85%></center> | ||
- | <br/><b>Fig 2.</b> 1 L culture incubated at 37oC till 0.5 nm optical density after inoculation with 5 mL of overnight. 2 mM IPTG induction for 2 hours. His-Tag Ni-NTA purification, centrifuged with Millipore 30kDa cutoff ultracentrifuge tubes. | + | <br/><b>Fig. 2.</b> 1 L culture incubated at 37oC till 0.5 nm optical density after inoculation with 5 mL of overnight. 2 mM IPTG induction for 2 hours. His-Tag Ni-NTA purification, centrifuged with Millipore 30kDa cutoff ultracentrifuge tubes. |
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<img src="https://static.igem.org/mediawiki/2012/6/6f/6-2%2C7-2_PUF-PIN_Protein_Purification.png" height=85% width=85%></center> | <img src="https://static.igem.org/mediawiki/2012/6/6f/6-2%2C7-2_PUF-PIN_Protein_Purification.png" height=85% width=85%></center> | ||
- | <br/><b>Fig 3.</b> 1 L culture incubated at 37oC till 0.5 nm optical density after inoculation with 5 mL of overnight. 2 mM IPTG induction for 2 hours. His-Tag Ni-NTA purification, centrifuged with Millipore 30kDa cutoff ultracentrifuge tubes. | + | <br/><b>Fig. 3.</b> 1 L culture incubated at 37oC till 0.5 nm optical density after inoculation with 5 mL of overnight. 2 mM IPTG induction for 2 hours. His-Tag Ni-NTA purification, centrifuged with Millipore 30kDa cutoff ultracentrifuge tubes. |
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- | <b>Fig 4.</b>In-Vitro Transcription with MEGAscript® T7 Kit (Invitrogen) | + | <b>Fig. 4.</b> In-Vitro Transcription with MEGAscript® T7 Kit (Invitrogen) |
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Revision as of 23:23, 30 September 2012