"
Team:UT-Tokyo/Project/H2 E.coli/Results
From 2012.igem.org
(Difference between revisions)
m (→Result) |
|||
| (10 intermediate revisions not shown) | |||
| Line 2: | Line 2: | ||
<!-- subpagename=の後の「ページ名」部分に、このページの名称を記述してください。 --> | <!-- subpagename=の後の「ページ名」部分に、このページの名称を記述してください。 --> | ||
<html> | <html> | ||
| - | </p><img id="abstimg" src="https://static.igem.org/mediawiki/2012/ | + | </p><img id="abstimg" src="https://static.igem.org/mediawiki/2012/d/d1/UT_Tokyo_P1010657.jpg" style="opacity:0.2;" alt="box-background image" /><p> |
<!-- ここから</p>の前までを編集してください --> | <!-- ここから</p>の前までを編集してください --> | ||
| - | + | Here our experimental results are given. | |
</p></div><p></html> | </p></div><p></html> | ||
<!-- 以下テンプレ部分まで自由記述 --> | <!-- 以下テンプレ部分まで自由記述 --> | ||
| - | == | + | == Results == |
| + | We measured the amount of H<sub>2</sub> produced by ''E.coli'' transformed with either “pLac-RBS-fhlA-d.term”(''fhlA'') or “pLac-RBS-fhlA(E363G)-d.term”(''fhlA'' (E363G)) by the method written in the page [[Team:UT-Tokyo/LabWork/AssayMethods|AssayMethods]]. <br /> | ||
| + | In this page, we describe the data. | ||
| - | + | The result is the following (Fig.1)<br /> | |
| - | + | ||
| - | The result is | + | |
[[file:UT Tokyo Fig diagram 640 480.png]] | [[file:UT Tokyo Fig diagram 640 480.png]] | ||
| - | Fig.1: | + | Fig.1:H<sub>2</sub>production in liquid LB medium containing the labeled glucose concentration.<br /> |
| - | + | The vertical axis expresses v/v% of H<sub>2</sub> in the gaseous phase after we cultivate each ''E.coli'' for 8 hours in a container with 1mL each of LB medium and the gaseous phase .The horizontal axis shows the kinds of ''E.coli'' and the concentrations of glucose in each medium(“LB+x%” means the glucose w/v concentrations of liquid LB medium is x% more than the usual medium.<br /> | |
| + | |||
The results shown by the Fig.1 is statistically sufficient to conclude the followings.<br /> | The results shown by the Fig.1 is statistically sufficient to conclude the followings.<br /> | ||
| - | + | *The amount of H<sub>2</sub> production is ''fhlA'' > ''fhlA'' (E363G) > wild type in these conditions. | |
| - | + | *There is a positive correlation between the amount of H<sub>2</sub> produced by ''fhlA'' and the glucose concentration in the liquid LB. | |
| - | + | ||
| - | The detailed interpretations of this result are done in the [[ | + | The detailed interpretations of this result are done in the [[Team:UT-Tokyo/Project/H2_E.coli/Discussion|Discussion]]. |
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
<!-- 以上自由記述 --> | <!-- 以上自由記述 --> | ||
| Line 69: | Line 33: | ||
{{:Team:UT-Tokyo/Template/Side|twdisp=block}} | {{:Team:UT-Tokyo/Template/Side|twdisp=block}} | ||
<!-- 以下サイドメニュー編集 ;の後には、もしこのページが第2階層にあれば、そのページ内の見出しを羅列。第1階層にあれば、そのカテゴリ内の第2階層ページ名を羅列する。;の後には名称を、:の後にはその説明を記述する。:と;のセットは適当に増減してください --> | <!-- 以下サイドメニュー編集 ;の後には、もしこのページが第2階層にあれば、そのページ内の見出しを羅列。第1階層にあれば、そのカテゴリ内の第2階層ページ名を羅列する。;の後には名称を、:の後にはその説明を記述する。:と;のセットは適当に増減してください --> | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
<!-- ここまでサイドメニュー編集を --> | <!-- ここまでサイドメニュー編集を --> | ||
<html></p> | <html></p> | ||
| Line 82: | Line 39: | ||
</div><p> | </div><p> | ||
</html> | </html> | ||
| - | {{:Team:UT-Tokyo/Template/Footer|prevname= | + | {{:Team:UT-Tokyo/Template/Footer|prevname=Home|prevfull=|nextname=Discussion|nextfull=Project/H2_E.coli/Discussion}} |
Latest revision as of 03:43, 27 September 2012
H2 E.coli: Results
Here our experimental results are given.
Results
We measured the amount of H2 produced by E.coli transformed with either “pLac-RBS-fhlA-d.term”(fhlA) or “pLac-RBS-fhlA(E363G)-d.term”(fhlA (E363G)) by the method written in the page AssayMethods.
In this page, we describe the data.
The result is the following (Fig.1)
Fig.1:H2production in liquid LB medium containing the labeled glucose concentration.
The vertical axis expresses v/v% of H2 in the gaseous phase after we cultivate each E.coli for 8 hours in a container with 1mL each of LB medium and the gaseous phase .The horizontal axis shows the kinds of E.coli and the concentrations of glucose in each medium(“LB+x%” means the glucose w/v concentrations of liquid LB medium is x% more than the usual medium.
The results shown by the Fig.1 is statistically sufficient to conclude the followings.
- The amount of H2 production is fhlA > fhlA (E363G) > wild type in these conditions.
- There is a positive correlation between the amount of H2 produced by fhlA and the glucose concentration in the liquid LB.
The detailed interpretations of this result are done in the Discussion.
