Team:HKUST-Hong Kong/Module

From 2012.igem.org

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           <a href="https://2012.igem.org/Main_Page"><img id="iGEM_Logo" class="Upper_Logos" src="https://static.igem.org/mediawiki/2012/3/32/Igem-logo.png"></a>
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           <a href="http://www.ust.hk"><img id="HKUST_Logo" class="Upper_Logos" src=""></a>
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          <a href="https://2012.igem.org/Team:HKUST-Hong_Kong"><img id="HKUST_iGEM_Logo" class="Upper_Logos" src="https://static.igem.org/mediawiki/2012/4/43/295197_446935765350523_472857901_n.jpg"></a>
           <div class="Navigation_Buttons" id="Team" align="center"><h3><p>TEAM</p></h3></div>
           <div class="Navigation_Buttons" id="Team" align="center"><h3><p>TEAM</p></h3></div>
           <div class="Navigation_Buttons" id="Project" align="center"><h3><p>PROJECT</p></h3></div>
           <div class="Navigation_Buttons" id="Project" align="center"><h3><p>PROJECT</p></h3></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Members">Members</a></p></div>
               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Members">Members</a></p></div>
               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Advisors">Advisors</a></p></div>
               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Advisors">Advisors</a></p></div>
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              <div class="Content_Buttons""><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Acknowledgement">Acknowledgement</a></p></div>
 
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           <div class="Navigation_Content" id="Project_Content">
           <div class="Navigation_Content" id="Project_Content">
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Project_Abstraction">Project Abstract</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Project_Abstraction">Abstract</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Background_and_Motive">Background and<br> Motive</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Background_and_Motive">Motive</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Project">Project Description</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Design_Overview">Design - Overview</a></p></div>
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              <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Module">Module</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Design_Module">Design - Module</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Chassis">Chassis</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Design_Chassis">Design - Chassis</a></p></div>
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              <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Safety">Safety</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Expectation">Expectation</a></p></div>
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              <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Potential_Application">Potential applicaiton</a></p></div>
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           </div>
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           <div class="Navigation_Content" id="Wet_lab_Content" align="center">
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Parts_and_Device">Parts and Device</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Parts_and_Device">Parts and Devices</a></p></div>
               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Notebook">Notebook</a></p></div>
               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Notebook">Notebook</a></p></div>
               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Characterization">Characterization</a></p></div>
               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Characterization">Characterization</a></p></div>
               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Achievement">Achievement</a></p></div>
               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Achievement">Achievement</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Prospect">Prospect</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Future_Work">Future Work</a></p></div>
           </div>
           </div>
           <div class="Navigation_Content" id="Human_practice_Content" align="center">
           <div class="Navigation_Content" id="Human_practice_Content" align="center">
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           </div>
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           <div class="Navigation_Content" id="Extras_Content" align="center">
           <div class="Navigation_Content" id="Extras_Content" align="center">
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                <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Medal_Requirements">Medal Requirements</a></p></div>
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                <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Safety">Safety</a></p></div>
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                <div class="Content_Buttons""><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Attribution">Attribution</a></p></div>
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                <div class="Content_Buttons""><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Acknowledgement">Acknowledgement</a></p></div>
               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Glossary">Glossary</a></p></div>
               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Glossary">Glossary</a></p></div>
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              <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Site_Map">Site Map</a></p></div>
 
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              <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Photo_Gallery">Photo Gallery</a></p></div>
 
           </div>
           </div>
         </div>
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<div><p align="center"><font size="20">PROJECT ABSTRACT</font></p></div>
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<div><p align="center"><font size="20">Module</font></p></div>
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               <h1><p align=center>Project name: B. hercules <br>---<i style="size:15px">The Terminator of Colon Cancer</i></p></h1>
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               <h1><p align=center>Target Binding Module</p></h1>
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           <p>The dispersal of toxic anti-tumor chemicals in the circulatory system during conventional cancer treatment prompts us to consider the need of alternative cancer therapies. In an effort to combat with colorectal carcinoma, we aim to use genetically modified <i>Bacillus subtilis</i> to execute targeted drug delivery to cancer cells in the digestive tract, offering an advantage of generating minimal adverse effect on normal colon epithelial cells. Targeting is achieved by expressing <i>RPMrel</i>, a colon tumor specific binding peptide, on the cell wall using a LytC cell wall binding system. The anti-tumor cytokine, bone morphogenetic protein 2(BMP-2), is synthesized and secreted out from the bacteria with the help of a signaling peptide fused to the protein. To control the timing and amount of BMP2 release, two regulatory systems, xylose-inducible system and ydcE/ydcD toxin-antitoxin system are introduced to minimize the harmful effect from BMP2 overdose. </p>
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           <p>Our decision to pursue colorectal carcinoma suppression arose from two key points obtained from preliminary research: 1) bone morphogenetic protein 2 (BMP-2) suppresses the growth of colon cancer cell growth <i>in vivo</i>, and 2) the phage display peptide RPMrel confers specific and preferential binding to non-differentiated colon cancer cells......<a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Module/Target_binding">Click to see more</a></p>
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          <div id="paragraph2" class="bodyParagraphs">
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              <h1><p align=center>Anti-tumor Molecule Secretion Module</p></h1>
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          <p>As our team objective is to provide a specific and efficient drug for Colon cancer, the way of drug synthesis and releasing is a significant part of our whole project. Hence this module is focusing on the production and delivery of anti-tumor drug......<a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Module/Anti_tumor">click to see more</a></p>
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          <div id="paragraph3" class="bodyParagraphs">
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              <h1><p align=center>Regulation and Control Module</p></h1>
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          <p>Our module aims at regulating our synthetic bacteria B. hercules. We first introduce a xylose inducible promoter, which can help us control the timing of BMP-2 expression. Our choice of xylose as an inducer stems from its induction efficiency, its little existence and low absorption rate in colon......<a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Module/Regulation_and_control">click here to see more</a></p>
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<p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong"><b>Home</b></a></p>
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</div>
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<div class="Sitemap_Content">
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<li><p><b>Team</b><p><ol>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Introduction">Introduction</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Supervisor">Supervisor</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Instructor">Instructor</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Members">Members</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Advisors">Advisors</a></p></li>
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</ol>
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</div>
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<div class="Sitemap_Content">
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<li><p><b>Project</b></p><ol>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Project_Abstraction">Abstract</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Background_and_Motive">Motive</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Design_Overview">Design - Overview</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Design_Module">Design - Module</a></p></li>
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<p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Module/Target_binding">Target Binding Module</a></p>
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<p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Module/Anti_tumor">Anti-tumor Molecule Secretion Module</a></p>
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<p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Module/Regulation_and_control">Regulation and Control Module</a></p>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Design_Chassis">Design - Chassis</a></p></li></ol>
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</div>
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<div class="Sitemap_Content">
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<li><p><b>Wet Lab</b></p><ol>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Parts_and_Device">Parts and Devices</a></p></li>
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<p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Parts_and_Device">Overview</a></p>
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<p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Construction">Construction</a></p>
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<p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Assembly">Assembly</a></p>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Notebook">Notebook</a></p></li>
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<p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Notebook/Logbook">Logbook</a></p>
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<p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Notebook/Protocol">Protocol</a></p>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Characterization">Characterization</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Achievement">Achievement</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Future_Work">Future Work</a></p></li></ol>
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</div>
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<div class="Sitemap_Content">
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<li><p><b>Human Practice</b></p><ol>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Overview">Overview</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Interview">Interview</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Presentation">Presentation</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Calendar">Calendar</a></p></li></ol>
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<div class="Sitemap_Content">
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<li><p><b>Extras</b></p><ol>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Medal_Requirements">Medal Requirements</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Safety">Safety</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Attribution">Attribution</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Acknowledgement">Acknowledgement</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Glossary">Glossary</a></p></li></ol>
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</div>
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Latest revision as of 00:53, 27 September 2012

Team:HKUST-Hong Kong - 2012.igem.org

Module

Target Binding Module

Our decision to pursue colorectal carcinoma suppression arose from two key points obtained from preliminary research: 1) bone morphogenetic protein 2 (BMP-2) suppresses the growth of colon cancer cell growth in vivo, and 2) the phage display peptide RPMrel confers specific and preferential binding to non-differentiated colon cancer cells......Click to see more

Anti-tumor Molecule Secretion Module

As our team objective is to provide a specific and efficient drug for Colon cancer, the way of drug synthesis and releasing is a significant part of our whole project. Hence this module is focusing on the production and delivery of anti-tumor drug......click to see more

Regulation and Control Module

Our module aims at regulating our synthetic bacteria B. hercules. We first introduce a xylose inducible promoter, which can help us control the timing of BMP-2 expression. Our choice of xylose as an inducer stems from its induction efficiency, its little existence and low absorption rate in colon......click here to see more

Retrieved from "http://2012.igem.org/Team:HKUST-Hong_Kong/Module"