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Team:HKUST-Hong Kong/Glossary
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<p><strong>Signal peptide</strong> – transient ‘zip code’ of premature protein which facilitates the secretion of protein in <em>Bacillus subtilis</em>.</p> | <p><strong>Signal peptide</strong> – transient ‘zip code’ of premature protein which facilitates the secretion of protein in <em>Bacillus subtilis</em>.</p> | ||
| - | <p><strong>YbdN</strong> – | + | <p><strong>YbdN</strong> – YbdN stated throughout this wiki refers to the first 24 amino acid residues of the protein YbdN in <em>Bacillus subtilis</em>, which fucntions as a signal peptide.</p> |
<p><strong><i>ybdN</i></strong> – Gene that codes for YbdN.</p> | <p><strong><i>ybdN</i></strong> – Gene that codes for YbdN.</p> | ||
<p><strong>YdjM</strong> – Signal peptide from protein YdjM in <em>Bacillus subtilis.</em></p> | <p><strong>YdjM</strong> – Signal peptide from protein YdjM in <em>Bacillus subtilis.</em></p> | ||
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<p><strong><i>lytC</i></strong> – Gene that codes for the LytC hydrolase.</p> | <p><strong><i>lytC</i></strong> – Gene that codes for the LytC hydrolase.</p> | ||
<p><strong>(EAAAK)<sub>n</sub> type helical linker</strong> – Stiff, long helical linker designed to separate fusion proteins with minimal disturbance to the function of both proteins. </p> | <p><strong>(EAAAK)<sub>n</sub> type helical linker</strong> – Stiff, long helical linker designed to separate fusion proteins with minimal disturbance to the function of both proteins. </p> | ||
| - | <p><strong>RPMrel</strong> – A nine amino acid disulfide-constrained peptide with the amino acid sequence n-CPIEDRPMC-c. Product of screening to isolate peptides with positive affinity for | + | <p><strong>RPMrel</strong> – A nine amino acid disulfide-constrained peptide with the amino acid sequence n-CPIEDRPMC-c. Product of screening to isolate peptides with positive affinity for HT-29 colon carcinoma cells.</p> |
<p><strong>FLAG™</strong> – 8 amino acid peptide tag trademarked by the Sigma-Aldrich Corp. used in attachment to the protein-of-interest to facilitate multiple assays.</p> | <p><strong>FLAG™</strong> – 8 amino acid peptide tag trademarked by the Sigma-Aldrich Corp. used in attachment to the protein-of-interest to facilitate multiple assays.</p> | ||
<p><strong><em>PxylA</em></strong> – The region containing the sigma A binding region for the xylose inducible promoter.</p> | <p><strong><em>PxylA</em></strong> – The region containing the sigma A binding region for the xylose inducible promoter.</p> | ||
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<p><strong><em>Ptms</em></strong> – A low efficient constitutive promoter in <em>E. coli</em> and <em>B. subtilis.</em></p> | <p><strong><em>Ptms</em></strong> – A low efficient constitutive promoter in <em>E. coli</em> and <em>B. subtilis.</em></p> | ||
<p><strong>EndoA</strong> – Endoribonuclease that can inactivate cellular mRNAs by cleaving them at specific but frequently occurring sites (i.e. UAUAAU↓AC).</p> | <p><strong>EndoA</strong> – Endoribonuclease that can inactivate cellular mRNAs by cleaving them at specific but frequently occurring sites (i.e. UAUAAU↓AC).</p> | ||
| - | <p><strong><i>ydcE</i></strong> – Gene that codes for the EndoA endoribonuclease.<br /></p> | + | <p><strong><i>ydcE</i> (<i>ndoA</i>)</strong> – Gene that codes for the EndoA endoribonuclease.<br /></p> |
<p><strong>YdcD</strong> – Antidote that counteracts the function of EndoA.</p> | <p><strong>YdcD</strong> – Antidote that counteracts the function of EndoA.</p> | ||
<p><strong><i>ydcD</i> (<i>endB</i>)</strong> – Gene that codes for the YdcD antidote. </p> | <p><strong><i>ydcD</i> (<i>endB</i>)</strong> – Gene that codes for the YdcD antidote. </p> | ||
Latest revision as of 23:18, 26 September 2012
Glossary
Signal peptide – transient ‘zip code’ of premature protein which facilitates the secretion of protein in Bacillus subtilis.
YbdN – YbdN stated throughout this wiki refers to the first 24 amino acid residues of the protein YbdN in Bacillus subtilis, which fucntions as a signal peptide.
ybdN – Gene that codes for YbdN.
YdjM – Signal peptide from protein YdjM in Bacillus subtilis.
ydjM – Gene that codes for YdjM.
BMP2 – Bone morphogenetic protein 2.
LytC – B. subtilis inherent cell-wall associated hydrolase mediating cell-wall turnover.
lytC – Gene that codes for the LytC hydrolase.
(EAAAK)n type helical linker – Stiff, long helical linker designed to separate fusion proteins with minimal disturbance to the function of both proteins.
RPMrel – A nine amino acid disulfide-constrained peptide with the amino acid sequence n-CPIEDRPMC-c. Product of screening to isolate peptides with positive affinity for HT-29 colon carcinoma cells.
FLAG™ – 8 amino acid peptide tag trademarked by the Sigma-Aldrich Corp. used in attachment to the protein-of-interest to facilitate multiple assays.
PxylA – The region containing the sigma A binding region for the xylose inducible promoter.
XylR – The transcriptional regulator for PxylA promoter.
Ptms – A low efficient constitutive promoter in E. coli and B. subtilis.
EndoA – Endoribonuclease that can inactivate cellular mRNAs by cleaving them at specific but frequently occurring sites (i.e. UAUAAU↓AC).
ydcE (ndoA) – Gene that codes for the EndoA endoribonuclease.
YdcD – Antidote that counteracts the function of EndoA.
ydcD (endB) – Gene that codes for the YdcD antidote.
Project
Wet Lab
Human Practice
