Team:HKUST-Hong Kong/Glossary

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           <a href="https://2012.igem.org/Main_Page"><img id="iGEM_Logo" class="Upper_Logos" src="https://static.igem.org/mediawiki/2012/3/32/Igem-logo.png"></a>
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           <a href="http://www.ust.hk"><img id="HKUST_Logo" class="Upper_Logos" src=""></a>
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          <a href="https://2012.igem.org/Team:HKUST-Hong_Kong"><img id="HKUST_iGEM_Logo" class="Upper_Logos" src="https://static.igem.org/mediawiki/2012/4/43/295197_446935765350523_472857901_n.jpg"></a>
           <div class="Navigation_Buttons" id="Team" align="center"><h3><p>TEAM</p></h3></div>
           <div class="Navigation_Buttons" id="Team" align="center"><h3><p>TEAM</p></h3></div>
           <div class="Navigation_Buttons" id="Project" align="center"><h3><p>PROJECT</p></h3></div>
           <div class="Navigation_Buttons" id="Project" align="center"><h3><p>PROJECT</p></h3></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Members">Members</a></p></div>
               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Members">Members</a></p></div>
               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Advisors">Advisors</a></p></div>
               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Advisors">Advisors</a></p></div>
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              <div class="Content_Buttons""><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Acknowledgement">Acknowledgement</a></p></div>
 
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           </div>
           <div class="Navigation_Content" id="Project_Content">
           <div class="Navigation_Content" id="Project_Content">
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Project_Abstraction">Project Abstract</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Project_Abstraction">Abstract</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Background_and_Motive">Background and<br> Motive</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Background_and_Motive">Motive</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Project">Project Description</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Design_Overview">Design - Overview</a></p></div>
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              <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Module">Module</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Design_Module">Design - Module</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Chasis">Chassis</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Design_Chassis">Design - Chassis</a></p></div>
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              <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Safety">Safety</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Expectation">Expectation</a></p></div>
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              <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Potential_Application">Potential applicaton</a></p></div>
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           </div>
           </div>
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           <div class="Navigation_Content" id="Wet_lab_Content" align="center">
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Characterization">Characterization</a></p></div>
               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Characterization">Characterization</a></p></div>
               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Achievement">Achievement</a></p></div>
               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Achievement">Achievement</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Prospect">Prospect</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Future_Work">Future Work</a></p></div>
           </div>
           </div>
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           <div class="Navigation_Content" id="Human_practice_Content" align="center">
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           <div class="Navigation_Content" id="Extras_Content" align="center">
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                <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Medal_Requirements">Medal Requirements</a></p></div>
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                <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Safety">Safety</a></p></div>
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                <div class="Content_Buttons""><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Attribution">Attribution</a></p></div>
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                <div class="Content_Buttons""><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Acknowledgement">Acknowledgement</a></p></div>
               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Glossary">Glossary</a></p></div>
               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Glossary">Glossary</a></p></div>
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              <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Site_Map">Site Map</a></p></div>
 
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              <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Photo_Gallery">Photo Gallery</a></p></div>
 
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          <div><p align="center"><font size="20">Glossary</font></p></div>
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          <p><strong>Signal peptide</strong> – transient &lsquo;zip code&rsquo; of premature protein which facilitates the secretion of protein in <em>Bacillus subtilis</em>.</p>
 +
<p><strong>YbdN</strong> – YbdN stated throughout this wiki refers to the first 24 amino acid residues of the protein YbdN in <em>Bacillus subtilis</em>, which fucntions as a signal peptide.</p>
 +
<p><strong><i>ybdN</i></strong> – Gene that codes for YbdN.</p>
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<p><strong>YdjM</strong> – Signal peptide from protein YdjM in <em>Bacillus subtilis.</em></p>
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<p><strong><i>ydjM</i></strong> – Gene that codes for YdjM.</p>
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<p><strong>BMP2</strong> – Bone morphogenetic protein 2.</p>
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<p><strong>LytC</strong> – <em>B.  subtilis </em>inherent cell-wall associated hydrolase mediating cell-wall turnover.</p>
 +
<p><strong><i>lytC</i></strong> – Gene that codes for the LytC hydrolase.</p>
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<p><strong>(EAAAK)<sub>n</sub> type helical linker</strong> – Stiff, long helical linker designed to separate fusion  proteins with minimal disturbance to the function of both proteins. </p>
 +
<p><strong>RPMrel</strong> – A nine amino acid disulfide-constrained peptide with the amino acid sequence n-CPIEDRPMC-c. Product of screening to isolate peptides with positive affinity for HT-29 colon carcinoma cells.</p>
 +
<p><strong>FLAG™</strong> – 8 amino acid peptide tag trademarked by the Sigma-Aldrich Corp. used in attachment to the protein-of-interest to facilitate multiple assays.</p>
 +
<p><strong><em>PxylA</em></strong> – The region containing the sigma A  binding region for the xylose inducible promoter.</p>
 +
<p><strong>XylR</strong> – The transcriptional regulator for  PxylA promoter.</p>
 +
<p><strong><em>Ptms</em></strong> – A low efficient constitutive promoter  in <em>E. coli</em> and <em>B. subtilis.</em></p>
 +
<p><strong>EndoA</strong> – Endoribonuclease that can inactivate cellular mRNAs by cleaving them at specific but frequently occurring sites (i.e. UAUAAU↓AC).</p>
 +
<p><strong><i>ydcE</i> (<i>ndoA</i>)</strong> – Gene that codes for the EndoA endoribonuclease.<br /></p>
 +
<p><strong>YdcD</strong> – Antidote that counteracts the function of EndoA.</p>
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<p><strong><i>ydcD</i> (<i>endB</i>)</strong> – Gene that codes for the YdcD antidote. </p>
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<p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong"><b>Home</b></a></p>
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</div>
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<div class="Sitemap_Content">
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<li><p><b>Team</b><p><ol>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Introduction">Introduction</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Supervisor">Supervisor</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Instructor">Instructor</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Members">Members</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Advisors">Advisors</a></p></li>
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</ol>
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</div>
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<div class="Sitemap_Content">
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<li><p><b>Project</b></p><ol>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Project_Abstraction">Abstract</a></p></li>
 +
<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Background_and_Motive">Motive</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Design_Overview">Design - Overview</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Design_Module">Design - Module</a></p></li>
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<p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Module/Target_binding">Target Binding Module</a></p>
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<p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Module/Anti_tumor">Anti-tumor Molecule Secretion Module</a></p>
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<p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Module/Regulation_and_control">Regulation and Control Module</a></p>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Design_Chassis">Design - Chassis</a></p></li></ol>
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</div>
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<div class="Sitemap_Content">
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<li><p><b>Wet Lab</b></p><ol>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Parts_and_Device">Parts and Devices</a></p></li>
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<p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Parts_and_Device">Overview</a></p>
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<p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Construction">Construction</a></p>
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<p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Assembly">Assembly</a></p>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Notebook">Notebook</a></p></li>
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<p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Notebook/Logbook">Logbook</a></p>
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<p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Notebook/Protocol">Protocol</a></p>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Characterization">Characterization</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Achievement">Achievement</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Future_Work">Future Work</a></p></li></ol>
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</div>
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<div class="Sitemap_Content">
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<li><p><b>Human Practice</b></p><ol>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Overview">Overview</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Interview">Interview</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Presentation">Presentation</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Calendar">Calendar</a></p></li></ol>
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</div>
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<div class="Sitemap_Content">
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<li><p><b>Extras</b></p><ol>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Medal_Requirements">Medal Requirements</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Safety">Safety</a></p></li>
 +
<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Attribution">Attribution</a></p></li>
 +
<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Acknowledgement">Acknowledgement</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Glossary">Glossary</a></p></li></ol>
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Latest revision as of 23:18, 26 September 2012

Team:HKUST-Hong Kong - 2012.igem.org

Glossary

Signal peptide – transient ‘zip code’ of premature protein which facilitates the secretion of protein in Bacillus subtilis.

YbdN – YbdN stated throughout this wiki refers to the first 24 amino acid residues of the protein YbdN in Bacillus subtilis, which fucntions as a signal peptide.

ybdN – Gene that codes for YbdN.

YdjM – Signal peptide from protein YdjM in Bacillus subtilis.

ydjM – Gene that codes for YdjM.

BMP2 – Bone morphogenetic protein 2.

LytC – B. subtilis inherent cell-wall associated hydrolase mediating cell-wall turnover.

lytC – Gene that codes for the LytC hydrolase.

(EAAAK)n type helical linker – Stiff, long helical linker designed to separate fusion proteins with minimal disturbance to the function of both proteins.

RPMrel – A nine amino acid disulfide-constrained peptide with the amino acid sequence n-CPIEDRPMC-c. Product of screening to isolate peptides with positive affinity for HT-29 colon carcinoma cells.

FLAG™ – 8 amino acid peptide tag trademarked by the Sigma-Aldrich Corp. used in attachment to the protein-of-interest to facilitate multiple assays.

PxylA – The region containing the sigma A binding region for the xylose inducible promoter.

XylR – The transcriptional regulator for PxylA promoter.

Ptms – A low efficient constitutive promoter in E. coli and B. subtilis.

EndoA – Endoribonuclease that can inactivate cellular mRNAs by cleaving them at specific but frequently occurring sites (i.e. UAUAAU↓AC).

ydcE (ndoA) – Gene that codes for the EndoA endoribonuclease.

YdcD – Antidote that counteracts the function of EndoA.

ydcD (endB) – Gene that codes for the YdcD antidote.

Retrieved from "http://2012.igem.org/Team:HKUST-Hong_Kong/Glossary"