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Team:UT-Tokyo/Project/H2 E.coli/Results
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We measured the amount of H₂ produced by E.coli transformed with either “pLac-RBS-fhlA-d.term”(fhlA) or “pLac-RBS-fhlA(E363G)-d.term”(E363G) by the method written in the page [[Team:UT-Tokyo/LabWork/AssayMethods|AssayMethods]]. In this page, we describe the data. | We measured the amount of H₂ produced by E.coli transformed with either “pLac-RBS-fhlA-d.term”(fhlA) or “pLac-RBS-fhlA(E363G)-d.term”(E363G) by the method written in the page [[Team:UT-Tokyo/LabWork/AssayMethods|AssayMethods]]. In this page, we describe the data. | ||
| - | + | The result is the following (Fig.1)<br /> | |
[[file:UT Tokyo Fig diagram 640 480.png]] | [[file:UT Tokyo Fig diagram 640 480.png]] | ||
Revision as of 22:50, 26 September 2012
H2 E.coli: Results
このページの概要を、簡単に記述。
Result
We measured the amount of H₂ produced by E.coli transformed with either “pLac-RBS-fhlA-d.term”(fhlA) or “pLac-RBS-fhlA(E363G)-d.term”(E363G) by the method written in the page AssayMethods. In this page, we describe the data.
The result is the following (Fig.1)
Fig.1:H₂production in liquid LB medium containing the labeled glucose concentration.
The vertical axis expresses v/v% of H₂ in the gaseous phase after we cultivate each E.coli for 8 hours in a container with 1mL each of LB medium and the gaseous phase .The horizontal axis shows the kinds of E.coli and the concentrations of glucose in each medium(“LB+x%” means the glucose w/v concentrations of liquid LB medium is x% more than the usual medium.
The results shown by the Fig.1 is statistically sufficient to conclude the followings.
- The amount of H₂ production is fhlA>fhlA(E363G)>wild type in these conditions.
- There is a positive correlation between the amount of H₂ produced by fhlA and the glucose concentration in the liquid LB.
The detailed interpretations of this result are done in the Discussion.
