Team:KAIST Korea/Project Background

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<li><b>Promoter</b> : <a href="링크걸어요">BBa_J23119</a></li>
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<li><b>Promoter</b> : <a href="http://partsregistry.org/Part:BBa_J23119">BBa_J23119</a></li>
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<li><b>RBS</b> : <a href="링크걸어요">BBa_B0034</a></li>
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<li><b>RBS</b> : <a href="http://partsregistry.org/Part:BBa_B0034">BBa_B0034</a></li>
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<li><b>att sites</b> : Recognition site for <a href="링크걸어요">BBa_K907000</a>(Mycobacteriophage Bxb1 integrase).</li>
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<li><b>att sites</b> : Recognition site for <a href="http://partsregistry.org/Part:BBa_K907000">BBa_K907000</a>(Mycobacteriophage Bxb1 integrase).</li>
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When <a href="링크 걸어요">BBa_K907000</a>(Mycobacteriophage Bxb1 integrase, Bxb1_Int) recognizes and inverts the sequence flanked with attB and attP sequences, promoter orientation is reversed, leaving recombined attL and attR sequences.  
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When <a href="http://partsregistry.org/Part:BBa_K907000">BBa_K907000</a>(Mycobacteriophage Bxb1 integrase, Bxb1_Int) recognizes and inverts the sequence flanked with attB and attP sequences, promoter orientation is reversed, leaving recombined attL and attR sequences.  
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Also, the signal can be restored to its initial state when <a href="링크 걸어요">BBa_K907000</a>/ <a href="링크 걸어요">BBa_K907001</a> (Mycobacterio-phage Bxb1 excisionase, Bxb1_Xis) complex recognizes and inverts the sequence flanked with attL and attR sequences back to original state.</br>
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Also, the signal can be restored to its initial state when <a href="http://partsregistry.org/Part:BBa_K907000">BBa_K907000</a>/ <a href="http://partsregistry.org/Part:BBa_K907001">BBa_K907001</a> (Mycobacterio-phage Bxb1 excisionase, Bxb1_Xis) complex recognizes and inverts the sequence flanked with attL and attR sequences back to original state.</br>
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Revision as of 22:12, 26 September 2012

KAIST Korea 2012 iGEM

Project : Overview


Our new biobrick part FlipFlop(BBa_K907002 and BBa_K907003) is a noble device which can generate binary signal as its name tells us.

This part is composed of three sub-parts.
  1. Promoter : BBa_J23119
  2. RBS : BBa_B0034
  3. att sites : Recognition site for BBa_K907000(Mycobacteriophage Bxb1 integrase).


Signal 0

The FlipFlop, initially, promotes the transcription and translation of down-stream gene, Protein A in the Figure 2, due to its promoter orientation.

When BBa_K907000(Mycobacteriophage Bxb1 integrase, Bxb1_Int) recognizes and inverts the sequence flanked with attB and attP sequences, promoter orientation is reversed, leaving recombined attL and attR sequences.


Signal 1

Then this device promotes the transcription and translation of upstream gene(must be designed in reverse orientation at construction step), or Protein B(Signal 1) in Figure 4.


Also, the signal can be restored to its initial state when BBa_K907000/ BBa_K907001 (Mycobacterio-phage Bxb1 excisionase, Bxb1_Xis) complex recognizes and inverts the sequence flanked with attL and attR sequences back to original state.


Signal 0
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